2.  To create a polycaprolactone mesh which enables cell activity and seeks to eventually provide an application in the field of tissue engineering toward biomimetic skin graft.

3.  Spun 4 more meshes › Chitosan included  Progress on cell work › Established cell line › Grew cells in tissue culture plates  Final Paper started › Materials and Method section

4.  1 wt.% Chitosan – Spun 3 meshes successfully  2 wt.% Chitosan – Spun 1 mesh successfully  Plan to also spin 0.5 wt.% chitosan

5. Date Mesh # Voltage (kV) Distance (cm) PCL Concentration (wt. % ) Chitosan Concentration (wt. % ) Syringe Pump flow rate (mL/min) 1/6/11010 150.02 2/7/111*15 0.02 2/15/112*15 0.02 2/15/11315 0.02 2/23/114*15 0Pipette 3/1/11515 0Pipette 3/1/116*15 0Pipette 3/11/1172015 0Pipette

6. Date Mesh # Voltage (kV) Distance (cm) PCL Concentration (wt. % ) Chitosan Concentration (wt. % ) Syringe Pump flow rate (mL/min) 3/11/11820 150Pipette 3/17/11915 100Pipette 3/17/11102015100Pipette 3/23/111115 200Pipette 3/23/11122015200Pipette 3/29/111315 1Pipette 4/7/11142015 1Pipette 4/11/1115 1Pipette 4/13/111615 2Pipette

7.  Worked with Gabi and Allison to establish cell line › Seeded mesh #4 with cells in tissue culture plate  Seeded mesh #6 with cells › Left over 2 weeks – fibroblast cells observed growing in plate

8.  Contamination (Fungus and Bacteria) › Observed in Mesh #4 – 1 st trial with cells with mesh › Possible solutions  increase sterilization time under UV light  Two trials of mesh in media with and without cells  Cells grew around the mesh in #6

9.  SEM issues › Unable to use the one at Janelia Farm › No contact from other university  Mesh too thick to see through on microscope › Possibly make thinner meshes to be able to see cells

10.  SEM images for meshes created  Cell work › Continue using different meshes (possibly try well plates)  Continue spinning PCL/chitosan solutions  Continue work for final symposium

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