1. Development and characterization of the common bean diversity panel (ADP)

2. BREEDING LOCALLY-ADAPTED PULSE CROPS FOR ENHANCED YIELD AND SEED QUALITIES: AN INTEGRATED, OUTCOME-BASED PLAN FOR ARS Karen Cichy ARSEast Lansing, MI Mike Grusak ARS Houston, TX Phil MiklasARSProsser, WA Talo Pastor-Corrales ARS Beltsville, MD Tim PorchARS Mayaguez, PR James BeaverUPR Mayaguez, PR Consuelo EstevezUPR Mayaguez, PR Gail WislerARS-NPL Roy ScottARS-NPL

3. The main goal of the project is to increase common bean production in Sub-Saharan Africa, through the development of germplasm resources, molecular tools, and scientific knowledge.

4. 1. Develop an Andean Diversity Panel (ADP) comprised of 300-500 dry bean lines for genetic characterization of targeted traits and identi- fication of breeding materials. 2. Identify and develop Andean germplasm with improved biological nitrogen fixation (BNF) and improved production on degraded low input soils. 3. Improve tolerance to abiotic stresses-drought and heat. 4. Identify Andean common bean cultivars with disease resistance genes for the control of the most widespread and yield-reducing diseases in target countries. OBJECTIVES

5. 5. Determine the phenotypic variation of seed phytic acid, flavonoids, and cooking time. 6. Determine the phenotypic variation and genetic control of seed mineral concentrations, pod mineral partitioning, and pod dry matter partitioning in Andean beans. OBJECTIVES

6. Objective 1: Develop Andean Diversity Panel

7. Increase of the ADP Panel Subsets Increased in: Prosser, Washington Isabela, Puerto Rico South Africa

8. Objective 2: Identification of ADP germplasm efficient under low fertility conditions

9. Morogoro, Tanzania

10. Trial of 95 landraces in Tanzania, 2012 Results from top 6 lines pods plant seeds podsd wtYield Shoot dry wtNoduleRustCBBALS Virus BCMV Sub IDNameNo. g 100 sdskg hagNo. TZ-4KILOMBERO 12.63.749.516557.4027.5 1.03.01.01.8 TZV-58KIJIVU 10.35.044.013737.3314.5 2.01.0 2.5 TZ-14KIANGWE 10.84.239.813665.209.0 1.02.03.01.0 TZV-73MASUSU 11.43.648.113348.2517.5 2.01.02.01.0 TZV-41MRONDO 14.94.637.413256.3114.3 1.0 TZ-7BUKOBA 15.85.336.413235.1220.8 1.02.01.51.0 Correlation 0.47**

11. Objective 3: Evaluate Abiotic Stress Response Top 25 performers for Drought – Othello, WA 2012 Drought Intensity Index = 40% Yield (kg ha -1 Geomean)

12. Objective 4: Identify disease resistant germplasm within the ADP Limited Common bacterial blight (CBB) Resistance in ADP Correlation coefficient between strains: 0.92 Lines 1-9 Scale, CBB Blue- 3353 Red- 484A

13. ADPSub-IDGenotype3353484ASAP6SU91 635BCV-508OAC Redstar4.02.5+- 430PR-4PR1013-34.52.5+- 432PR-6PR0637-1344.52.5+- 435PR-9RM-05-074.32.8+- 614BC-315ND0611064.03.0+- 32TZ-32RH No. 214.82.5+- 607BC-154NY 1054.83.0+- 96AF-1Rojo5.52.8+- 617BC-347Red Rider 9.08.0-- 618BC-351AC Elk 8.57.0-- 97AF-2Bilfa 4 9.08.8-- VAX 61.71.3++ Morales9.0 -- Mean8.67.7 LSD0.81.1 Response to strains 484A and 3353 of CBB 285 lines; 7 R., 16 Int., 262 S.

14. ADP Response to NL3 Strain of BCMNV Out of 303 lines: 37 with I gene and 3 with bc3 gene ADPSub-IDSourceGenotypeNL3 Response ENM- (bc3)SW13 (I) 614BC-315WM1131-159ND061106Necrosis(-)(+) 635BCV-508OT1127-57OAC RedstarNecrosis(-)(+) 607BC-154WM1131-157NY 105Necrosis(-)(+) 605BC-152WM1131-1451132-V96Necrosis(-) 118AF-23OT1127-26WernaNecrosis(-)(+) 427PR-1J.BeaverBadilloNecrosis(-)(+) 434PR-8J.BeaverPR0737-1Necrosis(-)(+) 121AF-26OT1127-30Kranskop HR-1Necrosis(-)(+) 225CC-2205IS-0227G 6415Necrosis(-)(+) 599BC-76OT1127-44IslesNecrosis(-)(+) 122AF-27OT1127-31KranskopNecrosis(-)(+) 113AF-18OT1127-17OPS-RS4Necrosis(-)(+) 126AFC-7 SELIAN 05Necrosis(+)(-) 632BCV-505OT1127-27TARS HT 1Necrosis(-)(+) 618BC-351WM1131-148AC ElkNecrosis(-)(+) 629BCV-502OT1127-15H9659-27-10Necrosis(-) 606BC-153WM1131-123NY 104Necrosis(-) 623BC-399WM1131-163DrakeNecrosis(-)(+) 624BC-401OT1127-55DollyNecrosis(-)(+) 630BCV-503OT1127-18H9659-23-1Necrosis(-) 26TZ-26OT1122-111Black WonderNecrosis(-) 51TZV-52OT1122-1RH No. 3Necrosis(-) 55TZV-56OT1122-16KABUKUNecrosis(-) 97AF-2OT1127-48Bilfa 4Necrosis(-) 117AF-22OT1127-24A483Necrosis(-) 123AF-28OT1127-33JennyNecrosis(-)(+) 452EC-6E.PeraltaINIAP 425Necrosis(-)(+) 456EC-10E.PeraltaINIAP 480Necrosis(-)(+) 517AG-102010-TP20Carioca, KibalaNecrosis(-)(+) 600BC-100OT1127-46K07921Necrosis(-)(+) 608BC-175WM1131-118UI-51Necrosis(-)(+) 609BC-248WM1131-146K-407Necrosis(-)(+) 610BC-265WM1129-121G-122Necrosis(-) 616BC-344WM1131-134OAC LyrickNecrosis(-)(+) 619BC-367WM1131-158UCD 0906Necrosis(-)(+) 622BC-378WM1131-112UCD 0701Necrosis(-)(+) 628BCV-501OT1127-14H9659-27-7Necrosis(-)(+)

15. Ashy stem blight response using Band-aid method Combined ResultsResponseLesion lengthNotes 18Res., 1-30-4 cm 155Int., 4-65-8 cm 88Susc., 7-9>= 9 cmor dead

16. Objective 5: Determine the phenotypic variation of seed phytic acid, flavonoids, and cooking time Cooking Time on 100 ADP lines

17. Elemental analysis of 40 Andean Entries – Seeds Elements Prosser Range Fold Range (Prosser) Ca (mg/g DW)0.7 – 2.74.1 x K (mg/g DW)11.3 – 19.91.8 x Mg (mg/g DW)1.4 – 2.92.1 x P (mg/g DW)4.2 – 6.81.6 x S (mg/g DW)1.9 – 2.91.5 x Cu (µg/g DW)6.5 – 10.81.7 x Fe (µg/g DW)63.2 – 99.61.6 x Mn (µg/g DW)10.9 – 19.61.8 x Mo (µg/g DW)0.4 – 4.611.0 x Ni (µg/g DW)0.6 – 4.77.8 x Se (µg/g DW)not detected Zn (µg/g DW)30.0 – 47.51.6 x Potential impact: Broad diversity exists for seed mineral concentrations. Association mapping with the ADP should lead to the identification of seed mineral QTLs for breeders. Objective 6: Determine the phenotypic variation and genetic control of seed mineral concentrations

18. Diversity Analysis - Based on 73 informative SNPs ADP Currently in the process of being SNP genotyped with 6,000 BeanCAP SNPs for AM Analysis

19. Sharing of ADP population Development of Access Database

20. Concluding Comments Initial findings in ADP – Limited disease resistance – Promising efficiency under low fertility, and for BNF – Useful range of phytonutrient concentration and cooking times ADP dataset will be important: – For mapping new traits/developing markers in Andeans – Establishing an initial baseline of African germplasm in research and breeding efforts – Determining research/breeding priorities focused on Africa Acknowledgements: