1. Target gene(s)Peak heightGene product functionEvidence of role in human cancer Otx2 61.6 Transcription factor, maintains H3K27 methylation 1, activates cell cycle and represses differentiation in medulloblastoma 2, enhances migration and proliferation of neuronal progenitor cells 3, association with Myc to induce gene expression in medulloblastoma 4, activates p53 pathway and senescence in medulloblastoma 5. Hypermethylated in breast cancer 6 and lung adenocarcinoma 7. Myo16 (Myr8, Nyap3)29.2 Nuclear myosin, expression linked to cell cycle progression - may regulate cell cycle progression 8. Interacts with Protein Phosphatase 1 (PP1) 9. Interacts with PI3K to activate PI3K, Akt, and Rac1 10. Interacts with WAVE1 complex 10. Endou (Pp11)24.1 Poly-U specific endonuclease.Overexpressed in ovarian adenocarcinomas 11. Xrra115.9 Modulates X-ray response 12. Ttc28 (Tprbk)14.5 Large protein with localisation linked and necessary for cell cycle progression 13. Interacts with AuroraB 13. Interacts with the BRCA1- containing BRCC complex 14. Expression dependent on p53 15. Knock down results in increased tumour growth 15. Gramd312.1 Upregulated upon senescence 16. Hpcal1 (Vilip3)12.1 Calcium sensor, nuclear translocation for neuronal differentiation 17. Galm11.7 Aldose-1-epimerase. Cass4 (Hepl)11.6 Poorly characterised, protein scaffold, other Cas proteins have roles in migration and chemotaxis, apoptosis, cell cycle, differentiation and progenitor cell function 18. Other Cas linked to poor prognosis and increased metastasis in cancer, as well as resistance to chemotherapeutics 18. Engase11.4 Endo-beta-N-acetylglucosaminidase, processing of free oligosaccharides in the cytosol 19 Parp1110.8 Uncharacterised, but other family members have roles in DNA damage response/repair and cell death 20. Inactivation of other family members enhances genomic instability and apoptosis inactivation 20. Stx6 (Syn6)10.3 Part of SNARE complex 21. Integrin trafficking, chemotactic cell migration 21. p53 target, required for cell adhesion and survival 22. Overexpressed in many types of human cancer 23. St8sia1 (Siat8)10.1 Component of sphingolipid metabolism pathways 24. Role in ER negative breast cancer 25, increased in cancer stem cells 26.

2. References for Table S8: 1: Bunt J, Hasselt NA, Zwijnenburg DA, Koster J, Versteeg R et al.. Acta Neuropathol. 2013 Mar;125(3):385-94 2: Bunt J, Hasselt NE, Zwijnenburg DA, Hamdi M, Koster J et al.. Int J Cancer. 2012 Jul 15;131(2):E21-32 3: Wortham M, Jin G, Sun JL, Bigner DD, He Y et al.. PLoS One. 2012;7(4):e36211 4: Bunt J, Hasselt NE, Zwijnenburg DA, Koster J, Versteeg R et al.. PLoS One. 2011;6(10):e26058. 5: Bunt J, de Haas TG, Hasselt NE, Zwijnenburg DA, Koster J et al.. Mol Cancer Res. 2010 Oct;8(10):1344-57. 6: Lian ZQ, Wang Q, Li WP, Zhang AQ, Wu L. Int J Oncol. 2012 Aug;41(2):629-38. 7: Rauch TA, Wang Z, Wu X, Kernstine KH, Riggs AD et al.. Tumour Biol. 2012 Apr;33(2):287-96 8: Cameron RS, Liu C, Pihkala JP. Cytoskeleton (Hoboken). 2013 Jun;70(6):328-48 9: Patel KG, Liu C, Cameron PL, Cameron RS. J Neurosci. 2001 Oct 15;21(20):7954-68 10: Yokoyama K, Tezuka T, Kotani M, Nakazawa T, Hoshina N et al.. EMBO J. 2011 Sep 23;30(23):4739-54. doi: 10.1038/emboj.2011.348. 11: Inaba N, Ishige H, Ijichi M, Satoh N, Ohkawa R et al.. Oncodev Biol Med. 1982;3(5-6):379-89. 12: Mesak FM, Osada N, Hashimoto K, Liu QY, Ng CE BMC Genomics. 2003 Aug 9;4(1):32. 13: Izumiyama T, Minoshima S, Yoshida T, Shimizu N. Gene. 2012 Dec 15;511(2):202-17. 14: Sowa ME, Bennett EJ, Gygi SP, Harper JW. Cell. 2009; 138:389–403. 15: Colleen A. Brady, Dadi Jiang, Stephano S. Mello, Thomas M. Johnson, Lesley A. Jarvis et al.. Cell. 2011 May 13; 145(4): 571–583. 16: Rovillain, E.; (2011) Doctoral thesis, UCL (University College London). 17: Jheng FF, Wang L, Lee L, Chang LS. Protein J. 2006 Jun;25(4):250-6. 18: Tikhmyanova N, Little JL, Golemis EA. Cell Mol Life Sci. 2010 Apr;67(7):1025-48. 19: Suzuki T, Yano K, Sugimoto S, Kitajima K, Lennarz WJ et al.. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9691-6. 20: Jean-Christophe Amé, Catherine Spenlehauer, Gilbert de Murcia. BioEssays Volume 26, Issue 8, pages 882–893, August 2004 21: Riggs KA, Hasan N, Humphrey D, Raleigh C, Nevitt C, Corbin D et al.. J Cell Sci. 2012 Aug 15;125(Pt 16):3827-39. 22: Zhang Y, Shu L, Chen X. J Biol Chem. 2008 Nov 7;283(45):30689-98. 23: Krista A. Riggs, Nazarul Hasan, David Humphrey, Christy Raleigh, Chris Nevitt et al.. Journal of Cell Science 2012 125, 3827–3839. 24: van Echten, G., and Sandhoff, K. (1993). J. Biol. Chem. 268:5341–5344. 25: Bobowski M, Vincent A, Steenackers A, Colomb F, Van Seuningen I et al.. PLoS One. 2013 Apr 23;8(4):e62559. 26: Liang YJ, Ding Y, Levery SB, Lobaton M, Handa K et al.. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):4968-73.