1. Information Don’t forget about your Microworlds Project! – Protist cultures on back counter – Yeast cells from lab today – Catch fruit flies in the back of the lab! – You will have a lot of time to work on microworlds in lab 9, 1 week before it is due LAB REPORT: – Make sure all the peer edits belong to you – Final draft due in TWO WEEKS

2. Lab 7: Cellular Respiration & Fermentation

3. Part 1: Alcoholic Fermentation Glucose Alcohol + CO2 + ATP

4. Part 1: Alcohol Fermentation Yeast + corn syrup CO2

5. Part 1: Alcohol Fermentation Test different amounts of yeast solutions – Clean tubes WELL and leave water inside soaking Design your own fermentation experiment – Different sugar substrates – pH buffers – Temperatures – Saline (salt) solutions

6. Part 2: Aerobic Respiration Design your own aerobic respiration experiment REMEMBER: Think about CELLULAR respiration, not lung-based respiration (smoker vs non-smoker is not a good choice)

7. Part 2: Aerobic Respiration Set up 2 beakers with bromothymol blue Blow through straw to see color change Add base to return beaker to control color

8. To collect your CO 2 produced, w/ a straw, blow into a beaker f/ 15 sec. Always try to blow about the same. Blowing should change the color. What is going on: CO 2 + H 2 O --> H 2 CO 3 carbonic acid gives one or more H+ ions to Bromothymol blue changing its color Add & count the drops of neutralizer needed to return the color to your starting point color. Part 2: Aerobic Respiration

9. The count of the drops of neutralizer is our measure of CO2 produced. The drops of neutralizer neutralize the CO2, returning the color to its starting color The more CO2 you blow out, the more neutralizer you'll need to return the color. Is there anyone who is unclear about how we are measuring CO2 production? Part 2: Aerobic Respiration