1. Fig 7.1

2. Fig 7.2

3. The bacteria flagella motor [source: Berg HC, Ann. Rev. Biochem 2003] Fig 7.3

4. Motors turn CCW RunTumble Motor turns CW When tethered to a surface the entire cell rotates, and Individual motors show two-state behavior time CCW CW Fig 7.4 Bacterial runs and tumbles are related to the rotation direction of theflagella motors. When all motors are CCW, the flagella turn in a bundle and cell is propelled. When one or more turn CW, the cell tumbles. Switching Dynamics of a single motor can be seen by tethering a cell to a surface by Means of its flagellum, so that the motor turns the entire cell body (at frequencies of a few hertz due to the large viscous drag of the body). 10 sec

5. Tumbling frequency 1/sec Time [min] Fig 7.5 : Tumbling-frequency of a population of cells exposed at time t= 5 to a step addition of saturating attractant (such as L-aspartate). After t=5, attractant is uniformly present at constant concentration. Exact adaptation means that the steady-state tumbling-frequency in the presence of attractant Is equal to that in its absence.

6. Fig 7.6 [Alon et al Nature 1999]

7. Fig 6.7

8. Figure 7.8: Activity dynamics in the fine-tuned model in response to a step addition of saturating attractant At time t=2 (dimensionless units throughout). A) Fine-tuned model shows exact adaptation with a tuned parameter set/ B) Dynamics when CheR level Is lowered by 20% with respect to fine-tuned parameter set. A time A a)b)

9. Fig 7.9 Robust mechanism for exact adaptation. Un-methylated receptors are methylated by CheR at a constant rate. Methylated receptors (marked with CH3 groups) transit rapidly between active and inactive states (the former marked with a star). Attractant binding increases the rate to become inactive, whereas repellents increase the rate to become active. De-mthylation is due to CheB, which acts only on the active methylated receptors. The active receptors catalyze the phosphorylation of CheY, leading to tumbles.

10. Fig 7.10: Activity dynamics in the robust model in response to addition of saturating attractant at time t=2. a) model parameters K=10, VR R =1, VB B=2. b) same parameters with R reduced by 20%. Exact adaptation is preserved, Steady-state tumbling frequency is fine-tuned. A A a) b) time

11. Fig 7.11. Experimental test of robustness in chemotaxis. The protein CheR was Expressed at different levels. Adaptation time and steady-state tumbling frequency varied with CheR, whereas adaptation remained exact. Adaptation precision is the ratio of tumbling frequency before and after saturating attractant (1mM aspartate). Wild-type tumbling frequency in this experiment is about 0.4/sec (black dot in b). Source: Alon, Barkai, Surette, Leibler, Nature 1999.