1. Drug Testing Notes, Poppy Seed Mythbusters Forensic Science 2/10/14

2. Drill What is your opinion of random drug testing at jobs? –Good idea or Invasion of Privacy?? –Choose one side and write a sentence to support your view.

3. Objectives IWBAT –Explain the screening and confirmation tests for drugs. –Explain whether eating poppy seeds will make you fail a drug test. NOTE: Wednesday, 2/11, Mysterious White Powder LAB – need closed toe shoes & hair ties

4. Agenda Drill Drug ID Notes Mythbusters: Poppy Seed Bagel Closure

5. Drug Identification The challenge of forensic drug identification comes in selecting analytical procedures that will ensure a specific identification of a drug. This plan, or scheme of analysis, is divided into two phases. –Screening test that is preliminary to reduce the possibilities to a manageable number. –Confirmation test that is a single test that specifically identifies a substance.

6. Preliminary Analysis Faced with the prospect that the unknown substance may be any one of a thousand or more commonly encountered drugs, the analyst must employ screening tests to reduce these possibilities to a small and manageable number.

7. Color Tests This objective is often accomplished by subjecting the material to a series of color tests that will produce characteristic colors for the more commonly encountered illicit drugs.

8. Type of TestWhat the Results Mean Marquis ColorOpiates – purple Amphetamines – orange- brown. Cobalt thiocyanate Cocaine – blue. Dillie-KoppanyiBarbiturates – violet-blue. VanUrkLSD – blue-purple. Duquenois- Levine Test Marijuana – purple.

9. Microcrystalline Tests Microcrystalline tests can also be used to identify specific drug substances by studying the size and shape of crystals formed when the drug is mixed with specific reagents.

10. Microcrystalline Tests CocaineMethamphetamine

11. Confirmational Determination Once this preliminary analysis is completed, a confirmational determination is pursued. Forensic chemists will employ a specific test to identify a drug substance to the exclusion of all other known chemical substances. Typically infrared spectrophotometry or gas chromatography-mass spectrometry is used to specifically identify a drug substance.

12. IR Spectrum of Cocaine

13. IR Spectrum of Novocaine

14. Collection and Preservation The field investigator has the responsibility of ensuring that the evidence is properly packaged and labeled for the laboratory. Generally common sense is the best guide, keeping in mind that the package must prevent the loss of the contents and/or cross-contamination.

15. Often the original container in which the drug was seized will suffice. All packages must be marked with information that is sufficient to ensure identification by the officer in the future and establish the chain of custody.

16. Demonstration of Screening Tests Video What is the purpose of the –positive control? –negative control? –sample? What test was used for …? and what color did it turn? Was that as expected? –amphetamine? –heroin? https://www.youtube.com/watch?v=hXh8pMvc 3-o&noredirect=1

17. Poppy Seed Bagel What do you think? Could eating a poppy seed bagel make you fail a drug test? Let’s find out! The worksheet is designed to follow the Scientific Method (hence the words in italics.)

18. Closure What is the Schedule and Classification of …? –Meth –Heroin –Cocaine –Marijuana –LSD –MDMA