1. Triglyceride Estimation
DR. BEENISH ZAKI
INSTRUCTOR DEPARTMENT OF BIOCHEMISTRY

2. OBJECTIVES
SKILL:
At the end of the practical session, the student shall be able to demonstrate the ability to estimate serum Triglycerides through quantitative method.
KNOWLEDGE
At the end of the session the student shall be able to explain and interpret the principle and its clinical application

3. Collection of sample: Serum sample is preferred.
A fasting specimen is not required but may be desired to decrease Lipemia.
Avoid hemolysis.

4. METHOD:
Most commonly used method for serum triglyceride estimation is enzymatic method by glycerol-phosphate oxidase method.

5. Principle : Triglycerides Lipase Glycerol + Fatty Acid
Glycerol + ATP Glycerokinase Glycerol 3 phosphate +ADP
Glycerol-3-phosphate Glycerol Phosphate oxidase Dihydroxyacetone-phosphate +H2O2
2H2O2 + 4-Aminoantipyrine Peroxidase
Quinine mine + HCl +H2O

6. Procedure: Take 1 cuvette. Label as Test.
With the blank provided calibrate the machine.
For Test mix well wait for 5 minute.
Read optical density using green filter

7. Procedure
Test
Serum
20µl
Reagent
2000µl

8. FORMULA O.D of Test x 200 = Concentration of test O.D of standard
The unit is mg/dl

9. NORMAL VALUE Triglycerides: <200mg/dl
Hypotriglyceridemia: < 60mg/dl

10. Cause of Hypertriglyceredimia
Diabetes Mellitus
Fatty Liver
Alcoholism
Starvation

11. Causes of Hypotriglyceredimia
Malabsorption Syndrome
PEM (protein energy malnutrition)

12. Any questions ?????

13. Thank you