1. Day 4: Glow Sticks Curiosity Zone The Magical World of Biology and Chemistry Dorcas Green, Arielle Darden (Today’s Reflection) Arielle Darden, Instructor – Weldon City, Schools,

2. Reflection…. Prompt: List one thing you learned, one thing you found interesting, and one thing you would like to learn more about/investigate further. Bodily Fluids pH /Determining unknown acidity and alkalinity. Blood Typing (A, B, AB, O) Arielle Darden, Instructor – Weldon City, Schools,

3. Blood Typing Clarification Arielle Darden, Instructor – Weldon City, Schools,

4. Agglutination A process that occurs within blood cells when the incorrect blood type is given to a patient. Simply referred to as clumping Arielle Darden, Instructor – Weldon City, Schools,

5. Who can give/receive blood? http://www.nobelprize.org/educational/medi cine/bloodtypinggame/ (Nobel Prize Site) http://www.nobelprize.org/educational/medi cine/bloodtypinggame/ Arielle Darden, Instructor – Weldon City, Schools,

6. Is it possible…. What is the meaning of the term ? Bioluminescence is the production and emission of light by a living organism. What organisms come to mind when you hear this term? Arielle Darden, Instructor – Weldon City, Schools,

7. How does it occur? One way is by the chemical reaction pigment luciferin. An enzyme known as luciferase oxidizes the pigment causing the glow effect. Show students the chemical reaction. Arielle Darden, Instructor – Weldon City, Schools Dorcas Green – NCSAS District-2 Co-director

8. Day 4: Glow Sticks Curiosity Zone The Magical World of Biology and Chemistry Dorcas Green, Arielle Darden (New Material for Today) Arielle Darden, Instructor – Weldon City, Schools Dorcas Green – NCSAS District-2 Co-director

9. Continued.. Another way is through genetic engineering with a protein known as GFP, Green Fluorescent Protein. Arielle Darden, Instructor – Weldon City, Schools,

10. GFP? Green Fluorescent Protein: -It was first isolated from a jellyfish Aequorea victoria.jellyfishAequorea victoria -It fluoresces when exposed to ultraviolet light Arielle Darden, Instructor – Weldon City, Schools Dorcas Green – NCSAS District-2 Co-director

11. Where do bioluminescent organisms dwell? On land(terrestrial) …. Arielle Darden, Instructor – Weldon City, Schools Dorcas Green – NCSAS District-2 Co-director

12. Where do bioluminescent organisms dwell? In water (marine environments) Arielle Darden, Instructor – Weldon City, Schools Dorcas Green – NCSAS District-2 Co-director

13. Day 4: Glow Sticks Curiosity Zone Lesson Investigation – Design Your Own Lab (DYOL) using glow sticks to illustrate how fireflies glow brighter on a warm summer night. Arielle Darden, Instructor – Weldon City, Schools Dorcas Green – NCSAS District-2 Co-director

14. Day 4: Glow Sticks Curiosity Zone Lesson Extension - Create a bioluminescent organism using modeling clay and glow sticks. Arielle Darden, Instructor – Weldon City, Schools,