1. 1 Origin of Life Created by Coach Blocker Schley County Middle School Ellaville, Georgia

2. 2 Aristotle (384 –322 BC) Proposed the theory of spontaneous generation  Idea that living things can arise from nonliving matter  Idea lasted almost 2000 years

3. 3 Spontaneous Generation  For centuries, people based their beliefs on their interpretations of what they saw going on in the world around them without testing their ideas  They didn’t use the scientific method to arrive at answers to their questions  Their conclusions were based on untested observations

4. Spontaneous Generation Abiogenesis- living organisms can arise spontaneously from non-living matter. 4

5. 5 Examples of Spontaneous Generation

6. 6 Example #1 Observation: Every year in the spring, the Nile River flooded areas of Egypt along the river, leaving behind nutrient-rich mud that enabled the people to grow that year’s crop of food. However, along with the muddy soil, large numbers of frogs appeared that weren’t around in drier times

7. 7 Example #1 Conclusion: It was perfectly obvious to people back then that muddy soil gave rise to the frogs

8. 8 Example #2 Observation: In many parts of Europe, medieval farmers stored grain in barns with thatched roofs. As a roof aged, it was not uncommon for it to start leaking. This could lead to spoiled or moldy grain, and of course there were lots of mice around.

9. 9 Example #2 It was obvious to them that the mice came from the moldy grain. Conclusion: It was obvious to them that the mice came from the moldy grain.

10. 10 Example #3 Observation: In the cities centuries ago, there were no sewers, no garbage trucks, no electricity, and no refrigeration. Sewage flowed down the streets. Chamber pots and left-over food were thrown out into the streets each morning. Many cities also had major rat problems and a disease called Bubonic plague.

11. 11 Example #3 Conclusion: Obviously, all the sewage and garbage turned into the rats Conclusion: Obviously, all the sewage and garbage turned into the rats.

12. 12 Example #4 Observation: Since there were no refrigerators, the mandatory, daily trip to the butcher shop, especially in summer, meant battling the flies around the carcasses (dead animals). Typically, carcasses were “hung by their heels,” and customers selected which chunk the butcher would carve off for them.

13. 13 Example #4 Obviously, the rotting meat that had been hanging in the sun all day was the source of the flies. Conclusion: Obviously, the rotting meat that had been hanging in the sun all day was the source of the flies.

14. 14 Disproving Spontaneous Generation

15. 15 Francesco Redi (1668) In 1668, Francesco Redi, an Italian physician, did an experiment with flies and wide- mouth jars containing meat

16. 16 Redi’s Experiment  Redi used open & closed flasks which contained meat.  His hypothesis was that rotten meat does not turn into flies.  He observed these flasks to see in which one(s) maggots would develop.

17. 17 Redi’s (1626-1697) Experiments Evidence against spontaneous generation: 1. Unsealed – maggots on meat 2. Sealed – no maggots on meat 3. Gauze – few maggots on gauze, none on meat

18. 18 Results of Redi’s Experiments The results of this experiment disproved the idea of spontaneous generation for larger organisms, but people still thought microscopic organisms like algae or bacteria could arise that way.

19. 19 Did Redi Use the Scientific Method?

20. 20 The Scientific Method Ask A Question, Hypothesis, Experiment, Analyze, Conclusion, Accept, Reject, or Modify the hypothesis

21. 21 Step 1 - Observation  There were flies around meat carcasses at the Butcher shop.  Where do the flies come from?  Does rotting meat turn into or produce rotting flies?

22. 22 Step 2 - Hypothesis If rotting meat is exposed to flies, then the adult flies will lay eggs in the rotting meat, because the maggots will use the rotting meat for food.

23. 23 Step 3 - Experimentation  Wide-mouth jars each containing a piece of meat were subjected to several variations of “openness” while all other variables were kept the same.  Control group — These jars of meat were set out without lids so the meat would be exposed to whatever it might be in the butcher shop.  Experimental group(s) — One group of jars were sealed with lids, and another group of jars had gauze placed over them.

24. 24 Step 4 - Data  Presence or absence of flies and maggots observed in each jar was recorded.  Control group – flies entered, laid eggs, & maggots emerged  Gauze covered – flies on gauze, but not in jar  Sealed jars – No maggots or flies on the meat

25. 25 Step 5 - Conclusion Only flies can make more flies. In the uncovered jars, flies entered and laid eggs on the meat. Maggots hatched from these eggs and grew into more adult flies. Adult flies laid eggs on the gauze on the gauze-covered jars. These eggs or the maggots from them dropped through the gauze onto the meat. In the sealed jars, no flies, maggots, nor eggs could enter, thus none were seen in those jars. Maggots arose only where flies were able to lay eggs. This experiment disproved the idea of spontaneous generation for larger organisms.

26. 26 Disproving Spontaneous Generation of Microbes

27. 27 Anton van Leeuwenhoek 1632-1723

28. 28 Anton van Leeuwenhoek (1674)  Leeuwenhoek began making and looking through simple microscopes  He examined pond water and observed tiny animals, fungi, algae, and single celled protozoa; “animalcules”  By end of 19 th century, these organisms were called microbes

29. 29 Leeuwenhoek’s Microscope

30. 30 John Needham (1745)  He showed that microorganisms flourished in various soups that had been exposed to the air  He claimed that there was a “life force” present in the molecules of all inorganic matter, including air and the oxygen in it, that could cause spontaneous generation to occur.

31. 31 Needham’s Results  Needham’s experiments seemed to support the idea of spontaneous generation.  People didn’t realize bacteria were already present in Needham’s soups.  Needham didn’t boil long enough to kill the microbes.

32. 32 Needham’s Experiment

33. 33 Lazzaro Spallanzani’s (1765)  Boiled soups for almost an hour and sealed containers by melting the slender necks closed  Boiled soups for almost an hour and sealed containers by melting the slender necks closed.  The soups remained clear.  Later, he broke the seals & the soups became cloudy with microbes.

34. 34 Spallanzani’s Results

35. 35Conclusion  Critics said sealed vials did not allow enough air for organisms to survive and that prolonged heating destroyed “life force”.  Therefore, spontaneous generation remained the theory of the time.

36. 36 The Theory Finally Changes

37. 37 How Do Microbes Arise?  By 1860, the debate had become so heated that the Paris Academy of Sciences offered a prize for any experiments that would help resolve this conflict.  The prize was claimed in 1864 by Louis Pasteur, as he published the results of an experiment he did to disproved spontaneous generation in microscopic organisms.

38. 38 Louis Pasteur (1822-1895)

39. 39 Pasteur's Problem  Hypothesis: If the dust in the air is not allowed into broth, then microbes will not exist in broth, because microbes live and feed on dust particles in the  Pasteur put broth into several special S-shaped flasks to trap dust particles.  Each flask was boiled and placed at various locations.

40. 40 Pasteur's Experiment - Step 1  Create S-shaped Flask - The special shape was intended to trap any dust particles containing bacteria.  Fill with broth

41. 41 Pasteur's Experiment - Step 2 Flasks boiled Flasks boiled Microbes Killed Microbes Killed

42. 42 Pasteur's Experiment - Step 3  Flask left at various locations  Did not turn cloudy  Microbes not found  Notice the dust that collected in the neck of the flask

43. 43 Pasteur's Experimental Results

44. 44 Pasteur’s S-shaped flask kept microbes out but let air in. Proved microbes only come from other microbes (life from life) - biogenesis biogenesis The Theory of Biogenesis

45. Review 45

46. 46 1668: Francisco Redi Conditions:Results: 3 jars covered with fine net No maggots 3 open jars Maggots appeared From where did the maggots come? What was the purpose of the sealed jars? Spontaneous generation or biogenesis?

47. 47 1745: John Needham Conditions:Results: Nutrient broth heated, then placed in sealed flask Microbial growth From where did the microbes come? Spontaneous generation or biogenesis?

48. 48 1765: Lazzaro Spallanzani Conditions:Results: Nutrient broth placed in flask, heated, then sealed No microbial growth Spontaneous generation or biogenesis?

49. 49 1861: Louis Pasteur Conditions:Results: Nutrient broth placed in flask, heated, not sealed Microbial growth Nutrient broth placed in flask, heated, then sealed No microbial growth Spontaneous generation or biogenesis?

50. Twenty years after Pasteur’s experiment, disproving spontaneous generation, Thomas Henry Huxley first used the term biogenesis. The concept of biogenesis is one of the most completely demonstrated principles in biology.

51. For life to come into being, scientists agree that four developments MUST have occurred. These include the following: 1. The formation of simple organic compounds important to life. (Amino Acids) 2. The formation of complex organic compounds. (Proteins) 3. The concentration and enclosure of these organic compounds. (Cell Membrane) 4. The linking of chemical reactions involved in growth. (Photosynthesis and Cellular Respiration)

52. The Heterotroph Hypothesis: In the 1930’s, the Russian scientist, A. I. Oparin, presented a hypothesis to explain how the first living thing might have developed. This theory was called the Heterotroph Hypothesis.

53. He proposed that:  Energy from ultraviolet light, lightning and volcanic heat caused chemical reactions to occur in the atmosphere.  These reactions created small organic molecules that dissolved in oceans.  Eventually, a large quantity of organic chemicals accumulated. (Amino Acids)  According to Oparin, the first life must have evolved from these organic compounds.

54.  In the 1950’s Stanley Miller tried to recreate conditions on Earth under which life may have formed. He used: -water to represent the oceans -Methane, Ammonia, Hydrogen and Water Vapor as the earth’s early atmosphere -electrical sparks as lightning.

56.  After his experiment, he found numerous organic compounds including amino acids. Although amino acids are the building blocks of protein, Miller had NOT created life.  He had shown that conditions like those on early Earth could produce some of the chemicals present in living things.