1. Perfused Bioreactor with Matrix-Enabled Capillary Scaffold (MECS) Team Members: Allyson Fry Bryan Gorman Jonathan Lin William Wong Advisor: Dr. John P. Wikswo A continuation from 2004-2005 (Barnett, Garrett, Harvill, Mayer, McClintock)

2. Objective Design a tissue engineering scaffold and supporting microfluidic control system to guide the development of a network of capillary tubes in vitro.

3. Introduction Bioreactor Capillary network Matrix-Enabled Capillary Scaffold (MECS) Perfusion

4. Motivation Simplified model for disease research Human system Control at many levels Variety of uses http://www.kids4research.org/mouse%20in%20hand%20facing%20left.jpg

5. Martins-Green   

6. Bioreactor Design Collagen MECS Supply Network Collagen Supply Network Support Filter

7. Overall Superstructure

8. Fractal pattern of vasculature

9. Matrix-Enabled Capillary Scaffold

10. Fabrication Strategy 1. Use two-layer photolithography to create mold of ‘egg crate’ design 2. Use soft lithography to create a PDMS stamp 3. Use stamp to deposit a sacrificial layer between two layers of collagen

11. Overall fluidics

12. Pumps and LabVIEW

13. Main Control Program pH sensor settings PID coefficients Graphs Histories of graph values Pump settings pH ranges

14. Main control program

15. Project Goals Model a complete microvascular network Support cellular processes Allow evaluation of molecular mechanisms Provide environment for endothelial cell differentiation Facilitate formation of stable tubular structures Support a flow of perfusate

16. Collaborators Dmitry Markov, PhDResearch advisor in microfabrication Lisa McCawley, PhDResearch advisor in tissue culture Philip SamsonResearch advisor in microfabrication Jason Greene Graduate student working on related bioreactor projects Walter GeorgescuAdvice on interfacing pumps with computer Ron ResisererClean room manager

17. References Martins-Green M, Li Q, Yao M. A New Generation Organ Culture Arising from Cross-Talk Between Primary Human Cell Types. Unpublished. Nanney, Lillian et al. Unpublished research. Tang MD et al. Fabrication of collagen gels that contain patterned micrometer- scale cavities. Adv. Mater, 2004.