1. Ｉｎｔｅｇｒａｔｉｎｇ Ｃｅｌｌｓ into Tissues
Sho Momota

2. CAM(Cell-adhesion molecules)
ECM(Extra cellular matrix)

3. ECM
Proteoglycans
Collagen fibers
Multiadhesive matrix proteins

4. CAM Cadherins Ig (immunoglobulin) super family Selectins Mucins
integrins

5. Cadherins Ca N-teminus Extra cellular region cell membrane
50-60% of the sequence is identical among different cadherins.
C-teminus
Cytoplasm

6. Types of cadherin More than 40 different cadherins are known
E-cadherin : Preimplantation embryos,
non-neural epethelial tissue
P-cadherin : Trophblast
N-cadherin : nervous system, lens, cardiac and
skeletal muscle

7. Character of cadherin Homophilic (like bind like)
E-cadherins bind to E-cadherins
P-cadherins bind to P-cadherins
E-cadherins usually not bind to P-cadherins

8. N-CAMs Group of Ca-independent cell-adhesion proteins
Ig super family of CAMs
Primarily Homophilic interaction
Encoded by a single gene.

9. Selectin Extravasation of leukocytes
Blood
Sialyl
Lewis-x
antigen
Extravasation of leukocytes
require the successive formation and breakage of cell-cell contacts between leukocytes in the blood and endothelial cells lining the vessels
Sugar binding lectin domain
P-selectin
Endothelial cell

10. Integrin Heterodimers of  and  subunits
Exhibit relatively low affinities for the ligands

11. Accelerates a formation of clot
IIb3 integrin
platelet
Accelerates a formation of clot
Collagen or thrombin
Conformational change of IIb3 integrin
Fibrinogen
platelet
Activated platelet

12. Ｄｅａｄｈｅｓｉｏｎ factors
Promote cell migration and can remodel the cell surface
Disintegrins
ECM
RGD sequence

13. De-adhesion factors
CELL
CAM
ECM
Disintegrins

14. Question?