1. Author(s): Michael Hortsch, Ph.D., 2010 License: Unless otherwise noted, this material is made available under the terms of the Creative Commons Attribution – Non-Commercial 3.0 License: http://creativecommons.org/licenses/by-nc/3.0/ We have reviewed this material in accordance with U.S. Copyright Law and have tried to maximize your ability to use, share, and adapt it. The attribution key found at the end of the module provides information about how you may share and adapt this material. Copyright holders of content included in this material should contact open.michigan@umich.edu with any questions, corrections, or clarification regarding the use of content. For more information about how to cite these materials visit http://open.umich.edu/education/about/terms-of-use. Any medical information in this material is intended to inform and educate and is not a tool for self-diagnosis or a replacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. Please speak to your physician if you have questions about your medical condition. Viewer discretion is advised: Some medical content is graphic and may not be suitable for all viewers.

2. Histology of Connective Tissue – Second Look Michael Hortsch, Ph.D. Department of Cell and Developmental Biology University of Michigan © 2010 Michael Hortsch

3. Name the areas indicated by the red, blue and green dashed lines and the combined red and green area.

4. Identify the tissue type shown in this H&E-stained tissue section.

6. Identify this tissue.

7. Identify this tissue type and also the type of protein that appears blue in this image. This tissue slide was stained with Masson’s Triple Stain/ Trichrome.

8. Identify the cell whose nucleus is marked by the green arrow.

9. Identify the cell in this EM micrograph and the protein matrix surrounding the cell.

10. Identify the extracellular structures in the above EM micrograph.

11. Name the major protein component you would expect to find in circle A and circle B, respectively. B A

12. Identify the cells indicated by the red arrows. What is their main secretion product ?

13. Identify the two cells labeled with A and B. A B

14. Identify the cells indicated by the red arrows. What is their main function ?

15. Identify the marked cells in this H&E- stained section of a lymph node. Inactive lymphocytes can be recognized as small cells with evenly stained basophilic nuclei and an almost invisible narrow cytoplasmic rim.

16. Identify the cells indicated by the green arrows. What is their main function ? Where are the stem cells located that produce these cells ?

17. Identify the cells indicated by the green arrows. What is their main secretion product and function ? This tissue slide was stained with PAS & Azure II.

18. Identify the cell in this EM micrograph.

19. Identify the structure in the green dashed circle.

20. Identify the round structure indicated by the red question mark. ?

21. Identify this tissue. Where would you find such tissue and what is its main function ?

22. Identify these network-like dark fibers. What is their main protein component ? This tissue slice was stained with silver

23. Identify the dark purple-stained structures, which are indicated by the white arrows. This tissue slide was stained with aldehyde fuchsin.

24. Slide 3:Michigan Histology 029-1_HISTO_40X.svs Slide 4:Michigan Histology 029-1_HISTO_40X.svs 100% Slide 5:Michigan Histology 250-1_HISTO_40X.svs Slide 6:Michigan Histology 112_HISTO_40X.svs Slide 7:Michigan Histology 250-2_HISTO_40X.svs Slide 8:Michigan Histology 029-1_HISTO_40X.svs Slide 9:Michigan Histology 025 - Fibroblast_001.svs Slide 10:Michigan Histology 026 - Connective tissue_001.svs 32% Slide 11:Michigan Histology 028 - Collagen & elastin_001.svs Slide 12:Michigan Histology 029-1_HISTO_40X.svs Slide 13:Michigan Histology 021 - Plasma cell_001.svs Slide 14:Michigan Histology 029-1_HISTO_40X.svs Slide 15:Michigan Histology 027_HISTO_40X.svs Slide 16:Michigan Histology 026_HISTO_40X.svs Slide 17:Michigan Histology160_HISTO_40X.svs Slide 18:Michigan Histology 020 - Mast cell_001.svs Slide 19:Michigan Histology 152_HISTO_40X.svs Slide 20:Michigan Histology 023 - Fat cells_001.svs Slide 21:Michigan Histology H2.svs from a developing fetus Slide 22:Michigan Histology 028-2_HISTO_40X.svs Slide 23:Michigan Histology 036_HISTO_20X.svs Attributions by Slide

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