1. Dot/slot-blot (a) - control (b) - treated
Dot Blot analysis of 75 SSH products. Each candidate was amplified from a plasmid clone using adaptor specific primers and 500ng of each amplicon arrayed on a nylon membrane. The blots were probed with (a) 200ng of labelled control mRNA and (b) 200ng of labelled treated mRNA (ECL Direct Nucleic Acid Labelling and Detection System, Amersham). Dr Kerry Barne

2. cDNA Macro/Microarray
Clontech Atlas Array (Nylon membrane)
cDNA array
(Glass slide)

3. DNA chip/oligoarray
Affymetrix chip
Scan

4. 4. Polymerisation completed
Real-time PCR R Q
2. Strand Displacement
3. Cleavage
4. Polymerisation completed
Forward
Reverse
Probe
1. Polymerisation
R = Reporter
Q = Quencher

5. ß-Actin ²Rn Vs Cycle Number
Real-time PCR
ß-Actin ²Rn Vs Cycle Number
0.2
0.4
0.6
0.8 1
1.2
1.4
1.6
1.8 2
2.2 10 20 30 40
cycle number
²Rn
no template
20000
10000
5000
2000
1000
500
100

6. Next generation platforms
PamGene 5D-Pulse™ Microarray Technology
Metrigenix (4D) Flow-Thru Chip™ Technology
PE Applied Biosystems 7900HT Micro Fluidic Card