1. Photosynthesis: An Overview

2.  The key cellular process identified with energy production is photosynthesis.  Photosynthesis is the process in which green plants use the energy of sunlight to convert water and carbon dioxide into high-energy carbohydrates and oxygen.

3. Investigating Photosynthesis ◦ Belgian Jan van Helmont conducted an experiment in the late 1600’s to better understand where a plants mass comes from. ◦ Planted a small willow tree in a container filled with soil. ◦ After 5 years of watering it, he found that the tree gained 160 pounds while the soil lost only two ounces. ◦ Concluded that most of the plants mass came from water, not the soil.

4. Investigating Photosynthesis Van Helmont’s experiment accounts for the “hydrate,” or water, portion of the carbohydrate produced by photosynthesis. But where does the carbon of the “carbo” portion come from? We now know that carbon dioxide in the air is also a major contributor of plant mass. In photosynthesis, the carbon in carbon dioxide is used to make sugars and other carbohydrates.

5. In 1772, Joseph Priestley made another important discovery. Placed a shoot of a green plant into a container of water. He then covered the container and lit a candle in it until it completely burned out. Later, Priestley was able to both burn the candle again and keep mice alive in the air. Priestley became the first person ever to observe the respiration of plants - the fact that they take in carbon dioxide and release oxygen. Also discovered how to dissolve CO 2 in water and invented the first soda pop. Investigating Photosynthesis

6. Jan Ingenhouz laid the groundwork for understanding photosynthesis when he demonstrated that green leaves absorb carbon dioxide and release oxygen only in sunlight. In darkness, he showed, leaves absorb oxygen and give off carbon dioxide, just like animals and non-green plant parts.

7. Photosynthesis

8. Light and Pigments ◦ What is the role of light and chlorophyll in photosynthesis?  How do plants capture the energy of sunlight? In addition to water and carbon dioxide, photosynthesis requires light and chlorophyll.  Plants gather the sun's energy with light-absorbing molecules called pigments.  The main pigment in plants is chlorophyll.  There are two main types of chlorophyll:  chlorophyll a  chlorophyll b

9. Light Visible spectrum – ROYGBIV Red = longest wavelength Violet = shortest wavelength Color of an object is determined by the color the object reflects Plants gather light using pigments

10. Light and Pigments  Chlorophyll absorbs light well in the blue-violet and red regions of the visible spectrum. Wavelength (nm) Estimated Absorption (%) 100 80 60 40 20 0 400 450 500 550 600 650 700 750 Chlorophyll b Chlorophyll a Wavelength (nm)

11. Light and Pigments  Chlorophyll does not absorb light well in the green region of the spectrum. Green light is reflected by leaves, which is why plants look green. Estimated Absorption (%) 100 80 60 40 20 0 400 450 500 550 600 650 700 750 Chlorophyll b Chlorophyll a Wavelength (nm)

12. Lights and Pigments Carotenoids are accessory pigments that allow plants to absorb light in mainly the blue and green regions of the spectrum and reflect red, yellow, and orange. These pigments are more prominent in the fall.

13. Light and Pigments  Light is a form of energy, so any compound that absorbs light also absorbs energy from that light.  When chlorophyll absorbs light, much of the energy is transferred directly to electrons in the chlorophyll molecule, raising the energy levels of these electrons.  These high-energy electrons are what make photosynthesis work.

14. Light and Pigments Plant pigments can be separated by a process called chromatography. Chromatography is used to separate mixtures of substances

15. The Photosynthesis Equation  The equation for photosynthesis is:  6CO 2 + 6H 2 O C 6 H 12 O 6 + 6O 2  carbon dioxide + water sugars + oxygen ◦ Glucose is the primary sugar produced by photosynthesis Light

16. The Photosynthesis Equation ◦ Photosynthesis uses the energy of sunlight to convert water and carbon dioxide into high-energy sugars and oxygen.

17. Inside a Chloroplast thylakoids – saclike photosynthetic membranes grana – stacks of thylakoids stroma – space outside the thylakoids

18. Overview of Photosynthesis photosynthesis occurs only in producers endergonic reaction – a chemical reaction that uses or stores energy Two steps of photosynthesis ◦ Light Reactions ◦ Calvin Cycle

19. Light Reactions require light use energy from light to produce ATP and NADPH occurs in the grana energy from “excited electrons” is used to bond ADP and P i to form ATP

20. Light Reactions 3 main events ◦ Light energy is absorbed by the chlorophyll and ATP is made ◦ Water is split into hydrogen ions, oxygen and electrons ◦ The hydrogen ions are used to make NADPH

21. Light Reactions Inputs: ◦ Light ◦ Water Outputs: ◦ ATP ◦ NADPH ◦ Oxygen (O 2 )

22. Calvin Cycle Also called the dark reactions or light- independent reactions process of synthesizing sugars occurs in the stroma does not require light converts CO 2 to sugar

23. Calvin Cycle 3-carbon sugars are produced ◦ Some used as energy sources ◦ Some converted to lipids, amino acids, parts of nucleic acids ◦ Two can combine to form 6-carbon sugars

24. Calvin Cycle Inputs: ◦ ATP ◦ NADPH ◦ CO 2 Outputs: ◦ Sugars

25. Photosynthesis Overview O2O2 CO 2 + H 2 0 Sugar ADP NADP + Light-Dependent Reactions (thylakoids) H2OH2O ATP NADPH Calvin Cycle (stroma) Light energy

26. In the end… a single 6-carbon sugar is formed from 6 molecules of carbon dioxide plants use the sugar for energy and to build more complex carbohydrates such as starches and cellulose other organisms eat the plants and use the energy stored in carbohydrates

27. Photosynthesis Overview

28. ◦ In van Helmont's experiment, most of the added mass of the tree came from  soil and carbon dioxide.  water and carbon dioxide.  oxygen and carbon dioxide.  soil and oxygen. Quiz

29. ◦ Plants use the sugars produced in photosynthesis to make  oxygen.  starches.  carbon dioxide.  protein. Quiz

30. ◦ The raw materials required for plants to carry out photosynthesis are  carbon dioxide and oxygen.  oxygen and sugars.  carbon dioxide and water.  oxygen and water. Quiz

31. ◦ The principal pigment in plants is  chloroplast.  chlorophyll.  carotene.  carbohydrate. Quiz

32. ◦ The colors of light that are absorbed by chlorophylls are  green and yellow.  green, blue, and violet.  blue, violet, and red.  red and yellow. Quiz