1. Ru–TAP complexes and DNA: from photo-induced electron transfer to gene photo-silencing in living cells
by Lionel Marcélis, Cécile Moucheron, and Andrée Kirsch-De Mesmaeker
Philosophical Transactions A
Volume 371(1995):
July 28, 2013
©2013 by The Royal Society

2. Reactions scheme for the PET process with [Ru(TAP)2(L)]2+ complexes (L=a bidentate ligand such as phenanthroline), followed by different possible reactions competing with each other.
Reactions scheme for the PET process with [Ru(TAP)2(L)]2+ complexes (L=a bidentate ligand such as phenanthroline), followed by different possible reactions competing with each other. In this scheme, the protonation of the reduced Ru–TAP complex and deprotonation of the G⋅+ moiety have been omitted. (L, phen or TAP, for example; PET, photo-induced electron transfer; BET, back electron transfer).
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

3. Structure of a Ru–TAP photo-adduct as determined by nuclear magnetic resonance spectroscopy [25].
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

4. Structure of (a) [Ru(HAT)2(phen)]2+, (b) [Ru(TAP)2(TPAC)]2+ (c) [Ru(phen/TAP)2(PHEHAT)]2+ (X=N for TAP and X=CH for phen).
Structure of (a) [Ru(HAT)2(phen)]2+, (b) [Ru(TAP)2(TPAC)]2+ (c) [Ru(phen/TAP)2(PHEHAT)]2+ (X=N for TAP and X=CH for phen).
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

5. Schematic of photo-cross-linking between two G-containing complementary ODNs by a free Ru–TAP complex.
Schematic of photo-cross-linking between two G-containing complementary ODNs by a free Ru–TAP complex.
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

6. Schematic of the inter-strand (duplex ODN) photo-cross-linking with the dinuclear complex [Ru(TAP)2(TPAC)Ru(TAP)2]4+.
Schematic of the inter-strand (duplex ODN) photo-cross-linking with the dinuclear complex [Ru(TAP)2(TPAC)Ru(TAP)2]4+. (Online version in colour.)‏
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

7. PAGE results of human telomeric sequence (T2AG3)4 and [Ru(TAP)2(TPAC)Ru(TAP)2]4+.
PAGE results of human telomeric sequence (T2AG3)4 and [Ru(TAP)2(TPAC)Ru(TAP)2]4+. Lane 1, non-illuminated sequence; lane 2, non-illuminated sequence in the presence of the complex; lanes 3–5, illuminated with an He/Cd laser at 442 nm in the presence of the complex for 3, 6 and 9 min, respectively. For lane 3, the approximate percentage for each photo-adduct is: (a) 28%, (b) 26%, (c) 16%, (e) 13%, (d,f) not measured.
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

8. Computer simulation of the intra-strand (human telomeric sequence) photo-cross-linking with the dinuclear complex [Ru(TAP)2(TPAC)Ru(TAP)2]4+.
Computer simulation of the intra-strand (human telomeric sequence) photo-cross-linking with the dinuclear complex [Ru(TAP)2(TPAC)Ru(TAP)2]4+. (Online version in colour.)‏
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

9. Schematic of the transformation of a coiled circular plasmid (or a covalently closed circular plasmid, CCC form) into an open circular plasmid (OC form, single-strand nick) and linear form (double-strand nick).
Schematic of the transformation of a coiled circular plasmid (or a covalently closed circular plasmid, CCC form) into an open circular plasmid (OC form, single-strand nick) and linear form (double-strand nick).
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

10. An example of the number of nodes of non-illuminated plasmids as detected by SFM.
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

11. The population distribution of plasmids (or fraction of molecules) plotted against the number of nodes for samples of untreated plasmids (filled squares) and for samples of enzymatically treated plasmids (filled circles, single-strand nick of plasmids).
The population distribution of plasmids (or fraction of molecules) plotted against the number of nodes for samples of untreated plasmids (filled squares) and for samples of enzymatically treated plasmids (filled circles, single-strand nick of plasmids).
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

12. The population distribution of plasmids after different times of illumination at 445 nm with [Ru(phen)2(PHEHAT)]2+, (a) up to 60 s illumination, (b) from 1 to 60 min illumination.
The population distribution of plasmids after different times of illumination at 445 nm with [Ru(phen)2(PHEHAT)]2+, (a) up to 60 s illumination, (b) from 1 to 60 min illumination. The direction of the arrows indicates the decrease or increase of the peaks.
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

13. The population distribution of plasmids after different times of illumination at 455 nm with [Ru(TAP)2(PHEHAT)]2+, (a) up to 60 s illumination, (b) from 1 to 60 min illumination.
The population distribution of plasmids after different times of illumination at 455 nm with [Ru(TAP)2(PHEHAT)]2+, (a) up to 60 s illumination, (b) from 1 to 60 min illumination. The direction of the arrows indicates the decrease or increase of the peaks and the shift of the maximum (horizontal).
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

14. (a) Non-illuminated plasmids treated with the enzyme EcoRI (restriction enzyme for a double-strand nick at a specific site).
(a) Non-illuminated plasmids treated with the enzyme EcoRI (restriction enzyme for a double-strand nick at a specific site). (b,c) Illuminated plasmids with the [Ru(TAP)2(PHEHAT)]2+ complex, treated afterwards with the enzyme EcoRI.
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

15. PAGE experiments with seven different 14-mer duplexes containing radiolabelled Ru–ODNs (black) and their G-containing complementary targets (light grey); lanes 1, non-illuminated; lanes 2, illuminated for 15 min with an He/Cd laser at 442 nm.
PAGE experiments with seven different 14-mer duplexes containing radiolabelled Ru–ODNs (black) and their G-containing complementary targets (light grey); lanes 1, non-illuminated; lanes 2, illuminated for 15 min with an He/Cd laser at 442 nm.
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

16. Schematic of the photo-induced inhibition of the replicative enzyme (DNA polymerase; grey crescent), functioning from a DNA template, starting at the primer, and owing to the photo-cross-linking of a Ru–ODN probe (black).
Schematic of the photo-induced inhibition of the replicative enzyme (DNA polymerase; grey crescent), functioning from a DNA template, starting at the primer, and owing to the photo-cross-linking of a Ru–ODN probe (black). In the dark, the enzyme works normally and the Ru–ODN probe is thus expelled.
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

17. Schematic of the different interaction for (a) [Ru(TAP)2(dppz′)]2+ (anchoring via dppz) and (b) [Ru(TAP)(TAP′)(dppz)]2+ (anchoring via TAP) in a duplex ODN.
Schematic of the different interaction for (a) [Ru(TAP)2(dppz′)]2+ (anchoring via dppz) and (b) [Ru(TAP)(TAP′)(dppz)]2+ (anchoring via TAP) in a duplex ODN.
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

18. PAGE experiments with radiolabelled Ru–ODNG conjugates irradiated with an He/Cd laser at 442 nm for increasing irradiation times, in the absence (lanes 1–6) and in the presence (lanes 7–12) of the complementary targets, and in the presence of two different ...
PAGE experiments with radiolabelled Ru–ODNG conjugates irradiated with an He/Cd laser at 442 nm for increasing irradiation times, in the absence (lanes 1–6) and in the presence (lanes 7–12) of the complementary targets, and in the presence of two different non-complementary targets (lanes 13 and 14).
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

19. Schematic of the competition between the ‘seppuku’ process (b) and the photo-cross-linking with a complementary sequence (a).
Schematic of the competition between the ‘seppuku’ process (b) and the photo-cross-linking with a complementary sequence (a).
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

20. The ODNs used with living cells for testing the photo-gene silencing in HPV+ SiHa cancer cells and tested in vitro in PAGE experiments.
The ODNs used with living cells for testing the photo-gene silencing in HPV+ SiHa cancer cells and tested in vitro in PAGE experiments. The Ru–ASO (a), the targeted complementary sequence for the E6 gene (b) and the Ru–nc conjugate (c).
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

21. Confocal microscopy: effect of the Ru–ASO treatment under illumination (2.5 h blue light radiation source at 380–480 nm, 4×24 W intense blue bulbs) on the restoration of production of protein p53, stained by specific p53 antibody (green). (a,b) Untreated ce...
Confocal microscopy: effect of the Ru–ASO treatment under illumination (2.5 h blue light radiation source at 380–480 nm, 4×24 W intense blue bulbs) on the restoration of production of protein p53, stained by specific p53 antibody (green). (a,b) Untreated cells non-illuminated and illuminated, respectively; (c,d) cells treated by Ru–nc non-illuminated and illuminated, respectively; and (e,f) cells treated by Ru–ASO non-illuminated and illuminated, respectively. (Online version in colour.)‏
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society

22. Antiproliferative effect of the Ru–ASO treatment under illumination (2
Antiproliferative effect of the Ru–ASO treatment under illumination (2.5 h blue light radiation source at 380–480 nm, 4× 24 W intense blue bulbs) on the organotypic HPV+ cell culture.
Antiproliferative effect of the Ru–ASO treatment under illumination (2.5 h blue light radiation source at 380–480 nm, 4× 24 W intense blue bulbs) on the organotypic HPV+ cell culture. Antigen used as a marker (Ki-67 immunohistochemical staining). (a,b) Cells treated by Ru–nc non-illuminated and illuminated, respectively; (c,d) cells treated by Ru–ASO non-illuminated and illuminated, respectively. (Online version in colour.)‏
Lionel Marcélis et al. Phil. Trans. R. Soc. A 2013;371:
©2013 by The Royal Society