1. 111/18/2015 Bacterial Genetics Filename BactGene.ppt

2. 211/18/2015 Background Concepts u Structure & Function of DNA & RNA u Genetic code u Transcription & translation u Protein synthesis u Gene Regulation: operators, promotors, repressors Filename: DNA.ppt

3. 311/18/2015 Lecture Outline u Genetic Flow u Genetic change u Transformation u Conjugation u Transduction u Plasmids u Transposons

4. 411/18/2015 Genetic Flow u Between Generations of Cells u Within Cells

5. 511/18/2015 Genetic Flow Between Generations

6. 611/18/2015 Genetic flow within a Generation

7. 711/18/2015 The Genetic Code u Genetic information is stored in DNA sequences found in u Chromosomes u copy/cell u DS- DNA u Circular u Plasmids

8. 811/18/2015 Plasmid u Smaller than chromosomal DNA u Contains only a few genes u independent of chromosome u circular u multiple copies!!

9. 911/18/2015 Griffith’s Transformation Exp. u Background u Pathogenic bacteria have capsule u Nonpathogenic bacteria have no capsule u Experiment: inject u live pathogens u live nonpathogens u dead pathogens u live pathogens+dead nonpathogens

10. 1011/18/2015 Bacterial Transformation

11. 1111/18/2015 Live Pathogens Mouse Dies recover pathogens

12. 1211/18/2015 Live non Pathogens Mouse lives Non pathogens recovered

13. 1311/18/2015 Dead Pathogens Mouse lives No Pathogens

14. 1411/18/2015 Live NonPathogens+ Dead Pathogens Live Pathogens!!

15. 1511/18/2015 Genetic Basis of transformation

16. 1611/18/2015 Recipient cell takes up DNA

17. 1711/18/2015 Recombination Occurs

18. 1811/18/2015 Bacterial Conjugation Sex and the single cell

19. 1911/18/2015 Bacterial Conjugation in E. coli

20. 2011/18/2015 Conjugation between F- & F+

21. 2111/18/2015 Replication & Transfer of F Factor

22. 2211/18/2015 F- cell converted to F+

23. 2311/18/2015 F factor & Chromosome Recombination

24. 2411/18/2015 F factor produces an Hfr

25. 2511/18/2015 Conjugation Between Hfr & F-

26. 2611/18/2015 Replication & Transfer

27. 2711/18/2015 Recombination in F- Cell

28. 2811/18/2015 Viral Transduction

29. 2911/18/2015 Phage Infects Donor Cell

30. 3011/18/2015 Bacterial Chromosome Broken Down

31. 3111/18/2015 Bacterial DNA Trapped in Phage Coat

32. 3211/18/2015 Bacterial DNA Infects New Cell

33. 3311/18/2015 Recombination can Occur

34. 3411/18/2015 Transposons u “Jumping Genes” u First discovered in corn u Genetic element u Moves other genes from place to place on the chromosome u Moved genes may be regulated differently

35. 3511/18/2015 Insertion Sequence “IS1”

36. 3611/18/2015 Complex Transposon

37. 3711/18/2015 R Plasmid “R1”