1. Paul Klapper Clinical Virology Manchester Royal Infirmary hepatitis C dried blood spot testing

5. - eluate – automated test system HCV antibody detection HCV antibody confirmatory testing - HCV RNA - HCV genotype dried blood spot

6. HCV antibody detection - sensitivity - 100% - specificity - 100% HCV antibody confirmatory testing - sensitivity 100% - specificity 100% how good is it? HCV RNA - sensitivity 96% - specificity 100% HCV genotype - sensitivity 96% - specificity 100% dried blood spot

8. HCV antibody detection - sensitivity - 100% - specificity - 100% HCV antibody confirmatory testing - sensitivity 100% - specificity 100% how good is it? HCV RNA - sensitivity 96% - specificity 100% HCV genotype - sensitivity 96% - specificity 100% [orasure 91.7%; 99.2%] [orasure - not available – 33-88%] dried blood spot

9. but that’s’ not all…. using same sample HBsAg – confirmation and full markers (sensitivity 96%) HBc Ab – (sensitivity 100%) HIV testing – HIV antibody/antigen and confirmation (2 additional assays) (sensitivity 98%) syphilis – good for infectious syphilis (sensitivity 100%) not so good (sensitivity 50%)for detection of syphilis at any time turnaround working toward 24 hr turnaround time – these samples a little longer (overnight elution) Web – secure server for results access dried blood spot