1. PL-ELS 2100 Ice

2. PL-ELS 2100 Ice Evaporation at Sub Ambient Temperatures The PL-ELS 2100 Ice comprises an Integrated Cooled Evaporator in combination with the ‘evaporation gas’ technology to provide sub-ambient operation The ‘evaporation gas’ is increased to compensate for the increase in relative humidity at sub-ambient temperatures This approaches enables the PL-ELS 2100 Ice to operate at sub-ambient temperatures and as low as 10°C.

3. Even at ambient temperature the response of semi-volatile, low-molecular weight analytes can be poor. So the PL-ELS 2100 Ice has been designed to operate at sub- ambient temperature to improve the detection of semi-volatile compounds Introduction

4. PL-ELS 2100 Ice Design Features Nebulisation Same nebuliser as PL-ELS 2100 Temperature Range: 25-90°C Evaporation Integrated Cooled Evaporator Tube Temperature range: 10-80°C Active Heating/Cooling Same evaporation gas technology as PL-ELS 2100

5. Peltier Cooled Evaporator Tube

6. PL-ELS 2100 Ice Design Feature for Sub-Ambient Operation Aqueous eluents can be removed at 15°C Organic solvents (e.g. THF) can be evaporated as low as 10°C The benefit of operating below room temperature provides maximum sensitivity for low-molecular weight semi-volatile compounds.

7. PL-ELS 2100 Ice Design Feature for Sub-Ambient Operation The PL-ELS 2100 Ice can heat or cool rapidly, which reduces equilibration time. 35°C to 10°C in 10 minutes 15°C to 80°C in 11 minutes Accurate temperature control of evaporator temperature eliminates external temperature effects

8. PL-ELS 2100 Ice Design Features Detection Same Optical Bench as PL-ELS 2100 Digital Output 24bit (10Hz) via RS232 Faster Time Constant (0.1 Secs) for UPLC

9. PL-ELS 2100 Ice FOR VOLATILE SOLUTES Sub-ambient Evaporator temperatures required - 10-30°C – PL-ELS 2100 Ice Gas flow optimised for sub-ambient temperatures and mobile phase – 100% Water @15°C requires 2.2SLM of gas Compounds with high vapour pressures, low MW pharmaceuticals, pesticides, hydrocarbons

10. Sub-Ambient Applications  The S/N ratio for thermally sensitive compounds such as Acetanilide is improved at sub-ambient temperatures  In addition, the response of compound’s with high boiling points and high volatility, such as Alkanes, is dramatically improved.  Hence, ELSD temperatures need to be as low as possible, to minimise volatility effects.

11. Sub-Ambient Applications: Acetanilide (MW 135)

12. Sub-Ambient Applications: Alkanes

13. Additional Benefits of the PL-ELS 2100 Ice Sub-ambient operation down to 10°C for maximum sensitivity to volatile compounds Active heating/cooling provides rapid equilibration between analyses. Improved accuracy of quantification.

14. Limit of Detection- PEG 106 x40 increase in S/N

15. What are you Missing?-Wine Analysis

16. PL-ELS 2100 Ice ‘Lock out’ Specs Sub-ambient operation down to 10°C High sensitivity to semi-volatile compounds DMSO transparency at ambient temperature Extremely low dispersion for high resolution separations Compatible with eluent flow rates, up to 5.0ml/min Rapid thermal equilibration Easy to use Extremely small footprint, stackable

17.  ELS2100Ice is the best choice for ELS detection for volatile and semi-volatile compounds.  ELS2100Ice has the widest range of sample detection for any ELSD, therefore it is the most ‘universal’ ELS Detector  ELS2100Ice is the only ELSD with operation at sub-ambient temperatures.  ELS2100Ice sub ambient specification can be used for lock- out specs vs competitive ELSDetectors. Key Points vs Competition

18. PL-ELS 2100/2100 Ice Specifications 200mm 450mm 200mm 415mm 450mm Light source: LED 480nm Detector: Photomultiplier tube Nebuliser temperature: ambient to 90°C in 1°C increments Evaporator temperature: PL-ELS 2100 Ice - 10 to 80°C (1°C increments) PL-ELS 2100 - ambient to 120°C (1°C increments) Gas flow rate: up to 3.25 SLM Gas pressure: typical 60-100psi, maximum 100psi Eluent flow rate: 0.2 to 5 ml/min Output: Analogue: 0-1V FSD Digital: 24bit, 10Hz using serial connection Communications: serial I/O (RS232), contact closure, TTL Power requirements: 90/120V AC or 220/250V AC 50/60 Hz Dimensions: 200x450x415mm (wxdxh) Part No.PL0860-0110 (110v), PL0860-0240 (240v)