1. GK-12 Saturday Workshop I October 16, 2010 RS: Amy Zimmerman CTs: Roy Center, Lee Kelly Genetic Transformation: Process and Applications

2. Why teach transformation??? Powerful tool used widely in research Apply scientific methods and concepts Introduce model organisms Hands-on laboratory investigation Real-world connections Standards based (*HS)

3. Overview of Lesson Module Genetic Transformation Lecture Modeling Transformation Activity Glowing Bacteria Laboratory Exercise Extension Activities Transformation Efficiency; Gene Therapy

4. Lesson Objectives Identify elements of good experimental design Create, analyze, and interpret data Execute basic microbiology techniques Understand the process and purpose of transformation Recognize real-life applications of biotechnology

5. Standards Adressed 7 th Grade Life Science Cell Biology 1a Genetics 2a,e Evolution 3b High School Biology Cell Biology 1a,c,d Genetics 5a,c,d*,e* Evolution 7a, 8a Investigation and Experimentation

6. Prior Knowledge Scientific Inquiry and Experimental Design Cell Structure and Function Membrane properties Genetics Genes, DNA, Proteins, Traits Bacteria Structure and reproduction

7. Implementation Timeline Day 1Genetic Transformation Lecture Day 2Modeling Transformation Activity Day 3Glowing Bacteria, Part 1 Day 4Glowing Bacteria, Part 2 Transformation Efficiency Day 5Gene Therapy Activity

8. Assessment Transformation success Handouts and lab write-up Extension activities