1. Experience with SARS Laboratory Diagnostics JSM Peiris, LLM Poon, Y Guan, KH Chan. The University of Hong Kong & Queen Mary Hospital

2. Early Response: March 28 I st Generation assays RT-PCR for P gene Indirect immuno-fluorescence serology using virus infected cells

3. 010,00020,00029,736 nt Orf1a polyprotein Orf1ab polyprotein E2 glycoprotein E1 protein M protein N protein RT-PCR tests: 1st Generation positivenegativeH20H20

4. 2 nd Generation RT-PCR tests April 03 Real Time RT-PCR Roche Light Cycler

5. Viral load in Nasopharyngeal aspirates Peiris et al 2003, Lancet 361: 1767-72

6. ABI GeneAmp 7000 using TaqMan detection Run capacity 96 specimens Run time 1.5 hrs Specificty- Probe Compare different gene targets P gene N gene

7. 10e5 10e6 10e4 10e3 10e2 10e1 water TaqMan assay for polymerase gene R 2 :0.99 10e6 10e5 10e4 10e3 10e2 10e1