1. What is AAALAC International?

2. What is AAALAC Int.? Accreditation and assessment for animal care and use programs. Accreditation and assessment for animal care and use programs. Completely voluntary and confidential programs. Completely voluntary and confidential programs. Open to all institutions that use animals in research, teaching or testing. Open to all institutions that use animals in research, teaching or testing. International in scope – more than 700 institutions in 29 countries are accredited. International in scope – more than 700 institutions in 29 countries are accredited.

3. What is AAALAC Int.? Private nonprofit organization. Private nonprofit organization. Governed by 60+ “Member Organizations,” (AVMA, ICLAS, AALAS, FELASA, etc.). Governed by 60+ “Member Organizations,” (AVMA, ICLAS, AALAS, FELASA, etc.). Programs are carried out by a 33-member Council on Accreditation and 170+ ad hoc Consultants. Programs are carried out by a 33-member Council on Accreditation and 170+ ad hoc Consultants. Administrative Offices are located in Rockville, Maryland (USA), Brussels, Belgium and Honolulu, Hawaii.

4. Some notes on the accreditation process Review process occurs every three years Review process occurs every three years The entire process is completely confidential. The entire process is completely confidential. There are opportunities to provide feedback and explanations to the site-visit team and the Council throughout the process. There are opportunities to provide feedback and explanations to the site-visit team and the Council throughout the process. AAALAC’s peer-review accreditation system is designed to be collegial and educational. AAALAC’s peer-review accreditation system is designed to be collegial and educational.

5. Why earn accreditation? It symbolizes quality. It symbolizes quality. It promotes scientific validity. It promotes scientific validity. It's a recruiting tool. It's a recruiting tool. It demonstrates accountability (to the public, etc.). It demonstrates accountability (to the public, etc.).

6. Why earn accreditation? It provides a confidential, external peer-review. It provides a confidential, external peer-review. It impresses funding sources and partners. It impresses funding sources and partners. It shows a real commitment to humane animal care. It shows a real commitment to humane animal care.

7. How does it work? Developing a “Program Description” (a document that details the entire animal care and use program). A care and use “program” encompasses: A care and use “program” encompasses: animal care and use policies and responsibilities animal care and use policies and responsibilities animal environment, housing and management animal environment, housing and management veterinary medical care veterinary medical care physical plant physical plant

8. Sources for Standards AAALAC standards are based on applicable local and national laws and regulations AAALAC standards are based on applicable local and national laws and regulations Guide for the Care and Use of Laboratory Animals (NRC 1996). Guide for the Care and Use of Laboratory Animals (NRC 1996). Animal Welfare Act (AWA) regulations Animal Welfare Act (AWA) regulations

9. How does it work? Submit application and Program Description to AAALAC Submit application and Program Description to AAALAC Schedule a program site visit review. Site visit teams include at least one Council member plus expert ad hoc Consultants. Schedule a program site visit review. Site visit teams include at least one Council member plus expert ad hoc Consultants. The site visit team submits their report to the full Council on Accreditation. The site visit team submits their report to the full Council on Accreditation. Council determines institution’s accreditation status. A letter detailing institution’s accreditation status is sent. Council determines institution’s accreditation status. A letter detailing institution’s accreditation status is sent.

10. Maintaining standards of animal care and use Getting it right

11. Role of the IACUC Protocol Review Protocol Review Program Review Program Review Facilities and Use Area Review Facilities and Use Area Review Address animal concerns Address animal concerns Note: The IACUC is required to conduct the same level of review twice per year as the AAALAC Int. Program review team conducts every three years. Note: The IACUC is required to conduct the same level of review twice per year as the AAALAC Int. Program review team conducts every three years.

12. IACUC Protocol Know your animal protocol. Know your animal protocol. Match to your grant application. Match to your grant application. Refer to the protocol often. Refer to the protocol often. Make no deviations from the protocol. Make no deviations from the protocol. There is no such thing as a pilot study that does not need IACUC approval. There is no such thing as a pilot study that does not need IACUC approval. Has everyone in the lab read the protocol and know where it is located? Has everyone in the lab read the protocol and know where it is located?

13. Training Prior to Work With Animals Training for research staff Training for research staff IACUC-required training includes RPF Modules, species specific training, and all required EHS training IACUC-required training includes RPF Modules, species specific training, and all required EHS training Hands on training by PI or designated staff member Hands on training by PI or designated staff member Training for animal care staff Training for animal care staff Required IACUC and EHS training Required IACUC and EHS training Documented on-the-job training Documented on-the-job training Certification by AALAS is ideal Certification by AALAS is ideal

14. Here? Or Here? Where would you expect better study results from ?

15. First Impressions  Keep laboratory neat and organized  This says a great deal about the lab  Convey image that animal users are interested in a quality program  Good Animal Care = Good Science

16. Storing Equipment & Supplies http://healthcare.spacesaver.com/centralsterile.jpg

17. Signage Animal related injury procedures Animal related injury procedures Emergency vet care Emergency vet care Rodent Survival Surgery Rodent Survival Surgery Reporting Concerns (animal welfare) Reporting Concerns (animal welfare)

18. Rodent Aseptic Surgery Must follow University of Pittsburgh published “Guidelines” Must follow University of Pittsburgh published “Guidelines” Appropriate sterilization of instruments Appropriate sterilization of instruments Surgical preparation of animal and surgeon Surgical preparation of animal and surgeon Monitor anesthesia Monitor anesthesia An anesthetized animal can not be left unattended An anesthetized animal can not be left unattended Post-Operative Care consistent with Post-Operative Care consistent with University of Pittsburgh “Guidelines” University of Pittsburgh “Guidelines” And as described in your protocol And as described in your protocol

19. Inhalant Anesthesia Use Use anesthetics only as described in the protocol. Use anesthetics only as described in the protocol. Gas anesthetic vaporizers should be calibrated at least every 2 years. Gas anesthetic vaporizers should be calibrated at least every 2 years. See policy at www.iacuc.pitt.edu. See policy at www.iacuc.pitt.edu.www.iacuc.pitt.edu Use a chemical fume hood or other means to scavenge gas anesthetic agents appropriately. Use a chemical fume hood or other means to scavenge gas anesthetic agents appropriately.

20. Documenting Procedures Blue Procedure Cards Research Records http://www.bedfordlabs.com/BedfordLabsWeb/products Dates, procedures, observations, initials

21. Animal Health Issues? Post-procedure complications. What do you do? Post-procedure complications. What do you do? What do you do with a sick animal on the weekend? What do you do with a sick animal on the weekend? Do you know the point at which the animal should be euthanized? What does the protocol state? Do you know the point at which the animal should be euthanized? What does the protocol state? Remember: ALL clinical care of animals MUST be coordinated through the Division of Laboratory Animal Resources (DLAR). Remember: ALL clinical care of animals MUST be coordinated through the Division of Laboratory Animal Resources (DLAR). Can you name a University Veterinarian or Vet Technician? Can you name a University Veterinarian or Vet Technician?

22. Clinical Records for Warm-blooded Species Other Than Rodents Each animal must have an individual clinical record Each animal must have an individual clinical record A post-procedure form (http://www.iacuc.pitt.edu) must be completed for every procedure done on the animal, as well as for euthanasia. A post-procedure form (http://www.iacuc.pitt.edu) must be completed for every procedure done on the animal, as well as for euthanasia.http://www.iacuc.pitt.edu For animals other than rodents, vital signs must be recorded at least every 15 minutes during surgery (including non-survival surgeries) on an anesthetic monitoring form (http://www.iacuc.pitt.edu ) For animals other than rodents, vital signs must be recorded at least every 15 minutes during surgery (including non-survival surgeries) on an anesthetic monitoring form (http://www.iacuc.pitt.edu )http://www.iacuc.pitt.edu

23. Clinical Records for Warm-blooded Species Other Than Rodents Completed clinical records must be turned in to DLAR Veterinary Services Active clinical records must be maintained in the animal housing room or procedure area Active clinical records must be maintained in the animal housing room or procedure area All drugs administered to an animal must be entered into its clinical record All drugs administered to an animal must be entered into its clinical record

24. Writing Legibly

25. Monitoring for Expired Drugs, Fluids & Materials http://www.patientpowernow.org/wp-content/uploads.jpg http://medrepexpress.com

26. Only use those drugs listed in the protocol Only use those drugs listed in the protocol Other drugs that may be needed for clinical care must be under the direction of a veterinarian Other drugs that may be needed for clinical care must be under the direction of a veterinarian Do you have a system for identifying and disposing of expired drugs? Do you have a system for identifying and disposing of expired drugs? Controlled substances will be audited. Controlled substances will be audited. Know the DEA laws!! Know the DEA laws!! DRUGS

27. Labeling Secondary Containers

28. Sharps Safety

29. Chemicals All chemical containers must be labeled All chemical containers must be labeled See “IACUC Policy on Secondary Labeling” See “IACUC Policy on Secondary Labeling” An expiration date must be on the label of all drug containers An expiration date must be on the label of all drug containers Keep chemicals away from human or animal food items Keep chemicals away from human or animal food items

30. http://www.mdp-miami.com/catalog/images/masksnmore Garbing Properly http://www.inmagine.com/searchterms/surgical_gloves.html

31. Occupational Health and Safety All animal users MUST be enrolled in the Animal Exposure Surveillance Program (AESP) All animal users MUST be enrolled in the Animal Exposure Surveillance Program (AESP) Physical Issues: Physical Issues: Do not recap needles- see EHS policy Do not recap needles- see EHS policy Bite wounds- do you know what to do? Bite wounds- do you know what to do? See animal related injury guidelines See animal related injury guidelines Personal Protective Equipment- do you know what is required? Personal Protective Equipment- do you know what is required?

32. Certifications Gas anesthetic vaporizers and anesthetic machines must be maintained and certified at least every two years Gas anesthetic vaporizers and anesthetic machines must be maintained and certified at least every two years Fume hoods and Biological Safety Cabinets must be certified annually Fume hoods and Biological Safety Cabinets must be certified annually

33. Animal transport Use only Approved transport routes Use only Approved transport routes No public elevators No public elevators Use only DLAR approved transport devices Use only DLAR approved transport devices

34. Visitors Policy Visitors to your lab need to obtain permission from the DLAR Clinical Director prior to their visit. To initiate permission to have a visitor, contact the DLAR at 412-648-8950. Visitors to your lab need to obtain permission from the DLAR Clinical Director prior to their visit. To initiate permission to have a visitor, contact the DLAR at 412-648-8950.

35. Animal Housing According to the “Guide”, no overcrowding is permitted According to the “Guide”, no overcrowding is permitted Review size requirements listed in the “Guide for the Care and Use of Laboratory Animals” for proper caging. Review size requirements listed in the “Guide for the Care and Use of Laboratory Animals” for proper caging. Delayed or incomplete weaning is the most common cause of overcrowded conditions Delayed or incomplete weaning is the most common cause of overcrowded conditions Identification Identification Cage cards must be visible and information completed Cage cards must be visible and information completed Animals must be identified Animals must be identified No housing of animals outside of the designated animal facility space is permitted unless approved by the IACUC No housing of animals outside of the designated animal facility space is permitted unless approved by the IACUC

36. Euthanasia AVMA Guidelines on Euthanasia - 2007 http://www.avma.org/issues/animal_welfare/euthanasia.pdf Your euthanasia procedure must be performed as written in your University of Pittsburgh protocol Your euthanasia procedure must be performed as written in your University of Pittsburgh protocol What methods do you use to ensure death? What methods do you use to ensure death? Physical methods of euthanasia require specialized training and justification Physical methods of euthanasia require specialized training and justification Use of a guillotine for euthanasia requires that it be properly maintained. Use of a guillotine for euthanasia requires that it be properly maintained.

37. University of Pittsburgh Policies Designed to help users and the University of Pittsburgh to maintain a program consistent with the “Guide for the Care and Use of Animals” Designed to help users and the University of Pittsburgh to maintain a program consistent with the “Guide for the Care and Use of Animals” Posted on the University of Pittsburgh IACUC website, www.iacuc.pitt.edu Posted on the University of Pittsburgh IACUC website, www.iacuc.pitt.eduwww.iacuc.pitt.edu If you need clarification or assistance, contact the IACUC office at 412-383-2008 If you need clarification or assistance, contact the IACUC office at 412-383-2008

38. Animal Care Acronyms to Know AAALAC-Int. -Association for the Assessment and Accreditation of Laboratory Animal Care-International AAALAC-Int. -Association for the Assessment and Accreditation of Laboratory Animal Care-International IACUC- Institutional Animal Care and Use Committee IACUC- Institutional Animal Care and Use Committee USDA- United States Department of Agriculture USDA- United States Department of Agriculture AWA- Animal Welfare Act AWA- Animal Welfare Act OLAW- Office for Laboratory Animal Welfare AALAS- American Association of Laboratory Animal Science OLAW- Office for Laboratory Animal Welfare AALAS- American Association of Laboratory Animal Science

39. www.iacuc.pitt.edu www.dlar.pitt.edu www.rcco.pitt.edu www.ehs.pitt.edu www.ibc.pitt.edu http://cmehttp://cme.hs.pitt.edu http://cme www.AAALAC.org

40. Please contact us if you have any questions IACUC Office 412-383-2008 IACUC@pitt.edu DLAR Main Office 412-648-8950DLAR@pitt.edu