1. School of Biomedical Engineering, Science & Health Systems WWW.BIOMED.DREXEL.EDU V 1.0 SD [040227] NANOSCALE BIOMECHANICS RESEARCH PROGRAM OVERVIEWPROGRAM OVERVIEW Faculty: Kenneth Barbee, PhD, Drexel University. E-mail: barbee@coe.drexel.edu Bioscope AFM Facility The Bioscope AFM facility supports many projects in BioNanoTechnology. It is used to measure surface topography, magnetic force, and mechanical properties of physical and biological materials. NanoFibers for Tissue Engineering Scaffolds The interactions between cells and surfaces play a key role in determining cell structure and function. Characterization and control of surface properties, therefore, can be used to optimize the adhesion, proliferation, and functional organization of cells in engineered scaffolds for the regeneration of tissue. Nanofiber scaffolds mimic the dimensions of natural connective tissue fibers.

2. School of Biomedical Engineering, Science & Health Systems WWW.BIOMED.DREXEL.EDU V 1.0 SD [040227] NANOSCALE BIOMECHANICS RESEARCH High Aspect Ratio Gold Nanotopography PROJECTOVERVIEWPROJECTOVERVIEW High aspect ratio gold nanotopography: cells do not adhere. 10 Microns Cells on Smooth Gold Controlling the adhesion of cells to surfaces is also important for the creation of patterned arrays of cells and hybrid biosensors. The nanotopography of the surface can be used to control cell adhesion. Faculty: Kenneth Barbee, PhD, Drexel University. E-mail: barbee@coe.drexel.edu

3. School of Biomedical Engineering, Science & Health Systems WWW.BIOMED.DREXEL.EDU V 1.0 SD [040227] NANOSCALE BIOMECHANICS RESEARCH Surface Topagraphy of Gelatin-coated Surface. PROJECTOVERVIEWPROJECTOVERVIEW Biological coatings, such as gelatin, not only provide specific binding sites for cell adhesion, but also provide a low aspect ratio roughness with nanoscale compliance. The AFM is also being used to measure the mechanical properties of biological thin films. Faculty: Kenneth Barbee, PhD, Drexel University. E-mail: barbee@coe.drexel.edu

4. School of Biomedical Engineering, Science & Health Systems WWW.BIOMED.DREXEL.EDU V 1.0 SD [040227] NANOSCALE BIOMECHANICS RESEARCH PROJECTOVERVIEWPROJECTOVERVIEW Magnetic Patterning of Particles and Ferrofluids 20 microns Height Phase Magnetic patterning of particles and ferrofluids is being used to create arrays of molecules, particles, and cells for a variety of applications. AFM and magnetic force miscroscopy (MFM) are used to characterize micromagnetic islands. Cobalt is evaporated onto the surface to create a 100 nm thick island (left). Magnetic domains are evident with random polarity (right). [add to slide 1]. Faculty: Kenneth Barbee, PhD, Drexel University. E-mail: barbee@coe.drexel.edu

5. School of Biomedical Engineering, Science & Health Systems WWW.BIOMED.DREXEL.EDU V 1.0 SD [040227] NANOSCALE BIOMECHANICS RESEARCH Magnetized Islands Attract Ferrofluid PROJECTOVERVIEWPROJECTOVERVIEW 6 Microns40 Microns When the islands are magnetized with a uniform field, they become polarized (left). Application of a magnetic field perpendicular to the surface causes a ferrofluid to be attracted to one pole of the island and repelled from the other. The ferrofluid can then be used as a mask for photolithographic applications. Faculty: Kenneth Barbee, PhD, Drexel University. E-mail: barbee@coe.drexel.edu

6. School of Biomedical Engineering, Science & Health Systems WWW.BIOMED.DREXEL.EDU V 1.0 SD [040227] NANOSCALE BIOMECHANICS RESEARCH When an external field is applied in the longitudinal direction, the space between the islands is masked. Application of An External Field in the Longitudinal Direction PROJECTOVERVIEWPROJECTOVERVIEW Faculty: Kenneth Barbee, PhD, Drexel University. E-mail: barbee@coe.drexel.edu