1. Eastern Africa Regional Laboratory Network (EARLN)- FMD Prepared By: S.N. Wekesa and C. Ngeleja (Coordinators EARLN-FMD)

2. EARLN-FMD 12 Countries and Contact Persons CountryName of AnimatorDesignition/Lab. Station KenyaDr. Sabenzia N. WekesaVet. FMDL/Coordinator TanzaniaDr. Chanasa Ngeleja MVet.CVL/Asst coordinator UgandaDr. Chris AyebazibweVet/ NADDEC EthiopiaDrs Gelagay/TesfayeVets/NVI/NAHDIC BurundiDr. Lazarus ButununguVet/Lab Sudan (N)Dr. M. HabielaVets/CVRLab Somalia Dr M Hassan Ali Jabra SAHSP National Epidemiologist S. SudanDr. David AjwokVet/admin RwandaDr David KiizaVet/Lab SomaliaDr. Mohamoud JabraVet Epidem/SAHSP Eritrea Dr. Tesfaalem Sebhatu Vet/NVL DjiboutiSaid Waiss MiguilTech/NLDAD DR CongoDr Bushu MulindaVet/Lab

3. EARLN-FMD

4. EARLN- FMD 2010 Support FAO, EuFMD 2010-12, Govts support First meeting in 2010 Sudan, Ethiopia, Somalia. Kenya, Uganda, Burundi, Tanzania (7 countries) Sharing information on country’s FMD activities Field-based foot and mouth disease outbreak investigation: – Biosecurity – using Participatory epidemiology (PE) tools – the importance of lesion aging for selection of animals for sampling and for establishing timeline of events

5. Participatory Epidemiology

6. Biosecurity

7. EARLN-FMD 2010 cont FMDV diagnosis – Window of detection by different techniques/tissues – Preservation and transportation The role of FMD Reference Centres (RCs) and lab networks in relation to Regional Roadmaps and the Progressive Control Pathway (PCP) for FMD Presentations and field activities – Nairobi and Nakuru Proficiency tests organized Kenya, Uganda, Ethiopia 2010 2011 – meeting in Ethiopia

8. Sampling technics to Vets and Technicians in Burundi Tanzania EUFMD/FAO RT-PCR (conv and rt) and IZSLER ELISA to all network member countries Batch 1 (N.Sud, Eth, Djb, Ken, Ug, Rwn, Bur, Tz) done Batch 2 to be conducted later Vaccine matching to Kenya (2 Vets trained in Nov.2011) Embakasi – virus isolation and conc, VNT & LPBE done, RT-PCR setup – partially completed Series of Nakuru Training Courses (NTC) to Kenya and other FMD free countries – 7 within 2 years EARLN-FMD Activities in 2011 - trainings

9. 2011 activities (cont) Regional newsletter FMD field and lab manual Monthly and annual reports Assessment of lab capacities Interactions network: e-mail, EUFMD Wiki-space, Phone calls and annual general meetings

10. Serotypes detected Country2009-20102011 N. SudanO and SAT 2 EthiopiaO and SAT 2 SomaliaO, A, SAT 1 and 2 KenyaO, A, SAT 1 and 2 BurundiO, A, SAT 1 and 2 No lab Dx prior 09 No vaccinations SAT 1 Vaccinations initiated UgandaA, O and SAT2O and SATs in wildlife TanzaniaA, O and SAT2???

11. Vaccine matching and used vaccines CountryOASAT 1SAT 2 Ken & Bur (10/11) OK77/78 LPBE 0.54 AK 5/80T155/71 (RHO VNT 0.51, 0.95 LPBE 0.75 0.25) K52/84 (K65/82 LPBE 0.11) EthNVI (local strains) X India (O manisa & A22 SudSerotype O/SUD/2008 (antigenically close to Manisa, BFS 1860 & Ind R2/75 using VNT at IAH-09 Bivalent O and SAT 2 UgTrivalent Vacc: O, SAT 1, SAT 2 (ex Kenya) Tz Vacc used undocm VNT Ind R2/75 (>1), Manisa (0.39), Taw98 (0.31) LPBE K77/78 (0.50) Manisa (0.53) Eritrea (0.37 & 0.52), A22 Irq (0.14 & 0.33) Sau 41/91 (0.19 & 0.38) Tur06 (0.1 & 0.32) Vac mat Eri (0.38)

12. Lab capacities in 2011 CountryBSLTests N.Sudan2Ag and Ab ELISA S. Sudan??ELISA, VNT Ethiopia2VI, Ag and Ab ELISA, PCR Somalia??VI, VNT Uganda2VI, Ag ELISA, SPBE Kenya2VI, Ag ELISA, VNT, NSP ELISA Tanzania2+Ag ELISA (IZSLER and IAH), LPBE, NSP ELISA, PCR (conventi. & rt)

13. Regional activities – Participated in regional meetings on PCP- Botswana March 2011 – Laboratory network activities – Networking with the EA Epidemiology network Collaborative projects and partners -EUFMD, FAO, WRL-Pirbright FMD control programmes in region – lab involvement – Surveillance, Diagnostics, Vaccine matching, vaccine quality assuarance. Regional Activities

14. Workplan for 2012  Continued support for network activities and meetings  Formation of research sub-groups within network  Sharing of information across networks in the region  Continuous training in lab. test  Support for upgrading of labs in the region and accreditation of a reference lab in EA  Wider participation in PTs  Support in acquisition of lab equipment and reagents

15. Workplan for 2012 Suggestions for presentation in Bangkok 2012 Results of Survey on Lab capacity in EA region Comprehensive Review of FMD Epidemiology in the EA region Activities of the EA network towards PCP the project to develop serotype-specific primers for EA FMD has been approved by EuFMD and withinin 12 months should evaluate primer sets for use in EA, (IAH and Lindholm, with local partners in Uganda and Tanzania. Actions: mainly IAH/Lindholm to initiate

16. Workplan for 2012 (cont) annual meeting (tentatively) the 5-6th March, in Nairobi (back to back with the FAO/OIE/AU regional PCP/Roadmap Development Workshop). Action: mainly from EuFMD - to confirm dates. the annual surveillance report for the OIE/FAO Annual Lab network report (on virus circulation in the EA region in 2011 - based on national confirmations and international ref lab findings) should be finalized in early Feb (the deadline is usually set by Pirbright, and allows for completion of lab results for outbreaks to the end of December). Action: Sabenzia/Chanasa

17. THANK YOU