1. Molecular Cell Biology Myosin Cooper

2. Diagram of a Sarcomere

3. Sliding Filament Model: Length-Tension Relationship Length (of the Sarcomere) Tension (Force produced by Muscle Contraction) Physiological Part of the Curve

4. Myosin Moving on Actin Filament in Muscle

5. ActoMyosin Cycle: ATP Hydrolysis & Movement

6. Structure of Myosin Head & Neck

7. Evidence for Twisting Head Model: Myosin Lever Arm Orientations

8. Thin Filament Proteins Drawn to Scale Tropomyosin Actin Troponin

9. Simple Steric Blocking Model Off On

10. Familial Hypertrophic Cardiomyopathy n Inherited human disease Heart dysfunction and sudden death n Autosomal dominant n Myosin heavy chain gene first n Other sarcomere components n Subtle mutations Mild effects on Biochemical Function

11. Myosin: Definitions n Biochemical: Actin-activated ATPase n Mechanical: Moves along actin filament n Structural: Conserved motor domain, with binding sites for actin and ATP

12. Structural Regions of Myosins

13. Phylogenetic Tree of Myosins

14. Myosin II: Contraction n Bipolar Filaments n Stress Fibers n Contractile Ring of Cytokinesis n Tail Contraction in Cell Migration Toothpaste Tube Model n Epithelial Cells Folding a Layer in Development Apoptosis - Extruding a Cell

15. Myosin-V: Hand-over-hand Processivity

16. Myosin-V: Molecular Movement Atomic Force Microscopy

17. Tapping Mode

18. Myosin V Auto-Inhibition

19. Myosin V in Budding Yeast Tracks of Actin Filaments... Anchored via Barbed Ends At Specific Locations on the Plasma Membrane (1) Filaments Bundled (2)

20. Myosin V in Budding Yeast Cargos for the Myosin... Secretory Vesicles for Polarized Growth (3) Distal Plus Ends of Mts, to Position the Mitotic Spindle (4) mRNA to Specify Daughter-Cell Property (5) Inheritance / Segregation of Organelles, e.g. Vacuole/Lysosome (6)

21. n Moves Backward - Toward Pointed End n Motor domain has insertion sequence in position similar to an insertion in Ncd, the backwards kinesin n Associated w/ Unusual Actin Filament Structures during Spermatogenesis in Drosophila and C. elegans Myosin VI

26. ControlAfter Intense Sound Tip Links by Scanning EM: Effect of Sound

27. External Swf Files n Channel Gating n Positive adaptation n Negative adaptation

28. Myosin Isoform Localization IcVIVIIa

29. Myo1c Localization by ImmunoEM

30. NMB-ADP Analogue Strategy for Inhibiting an ATPase

31. Electrophysiology Results

32. Model Wild-type Mutant

33. End