1. PV92 Insert Lab Make sure the gels are in lab note books! I sent them to pingry email on Friday. Print out now! After presentation mark genotype of each person that has a lane.

2. All Alus are approximately 300 bp in length and derive their name from a single recognition site for the restriction enzyme AluI located near the middle of the Alu element. Human chromosomes contain about 1,000,000 Alu copies, which equal 10% of the total genome. Alu elements probably arose from a gene that codes for RNA signal peptide for export

3. Alu Insert Alu elements are found only in primates – the "monkey" branch of the evolutionary tree, which includes humans. So, all of the hundreds of thousands of Alu copies have accumulated in primates since their separation from other vertebrate groups about 65 million years ago. Once an Alu inserts at a chromosome locus, it can copy itself for transposition, but there is no evidence that it is ever excised or lost from a chromosome locus. So, each Alu insertion is stable through evolutionary time. Each is the "fossil" of a unique transposition event that occurred only once in primate evolution. Like genes, Alu insertions are inherited in a Mendelian. An estimated 500-2,000 Alu elements are restricted to the human genome. The vast majority of Alu insertions occur in non-coding region. However, an Alu insertion in the NF-1 gene is responsible for neurofibromatosis I.

4. Determine the Genotype of Each Student ++. +-, --

5. Genotypes of Students