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DISCOVERY OF CYTOKININS SEARCH FOR CELL DIVISION FACTORS THAT WOULD ALLOW PLANT TISSUES TO DIVIDE IN TISSUE CULTURE CELL DIVISION ACTIVITY IDENTIFIED IN.

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Presentation on theme: "DISCOVERY OF CYTOKININS SEARCH FOR CELL DIVISION FACTORS THAT WOULD ALLOW PLANT TISSUES TO DIVIDE IN TISSUE CULTURE CELL DIVISION ACTIVITY IDENTIFIED IN."— Presentation transcript:

1 DISCOVERY OF CYTOKININS SEARCH FOR CELL DIVISION FACTORS THAT WOULD ALLOW PLANT TISSUES TO DIVIDE IN TISSUE CULTURE CELL DIVISION ACTIVITY IDENTIFIED IN COCONUT MILK CELL DIVISION ACTIVITY ALSO FOUND IN VASCULAR TISSUES ADENINE FOUND TO BE SLIGHTLY ACTIVE OLD BOTTLE OF DNA FOUND TO HAVE STRONG ACTIVITY, BUT NOT NEW BOTTLE KINETIN IDENTIFIED AS DEGRADATION PRODUCT OF DNA

2 KINETIN (6-furfurylaminopurine) (Discovered as a cell division factor)

3 STRUCTURE OF BENZYLADENINE (BA) - ANOTHER SYNTHETIC CYTOKININ

4 trans-Zeatin (The Naturally Occurring Cytokinin)

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6 Biological isoprene unit

7 ( active form)

8 CYTOKININ DEGRADATION

9 Tobacco plants overexpressing gene for cytokinin oxidase EFFECT OF CYTOKININ DEFICIENCY ON GROWTH OF TOBACCO PLANTS WILD TYPE

10 Wild-type Tobacco

11 Tobacco overexpressing cytokinin oxidase

12 Wild-type CO Overexpressor

13 Wild- type root tip

14 CO Overexpressor root tip  Cytokinin inhibits root growth

15 INCREASING IAA CONCENTRATION INCREASING CK CONCENTRATION

16 OVEREXPRESSION OF Cyclin D3 (CYCD3) BYPASSES THE CYTOKININ REQUIREMENT FOR CELL DIVISION

17 Crown gall tumor on tomato stem (Agrobacterium tumefaciens)

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19 TWO MAJOR OPINES FOUND IN CROWN GALL TUMORS GENES FOR OPINE SYNTHESIS PRESENT ON T-DNA FROM AGROBACTE RIUM

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21 Witches’ broom on balsam fir (Corynebacterium fascians)

22 Protonemal filaments of the moss, Funaria. (Control)

23 Protonemal filaments of the moss, Funaria. (Treated with Benzyladenine)

24 BUD FORMATION IN THE MOSS, Funaria

25 EFFECT OF CYTOKININ ON LEAF SENESCENCE CYTOKININ- TREATED

26 CYTOKININ CAUSES CELLS TO BECOME SINKS

27 CYTOKININ INHIBITS SENESCENCE

28 CYTOKININ PROMOTES EXPANSION OF RADISH COTYLEDONS

29 CYTOKININ SIGNALING INVOLVES A TWO- COMPONENT SYSTEM

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