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Published byVeronica Wilson Modified over 8 years ago
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Microscopes & Lab Techniques
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What is a light microscope? Light Microscope – a device that uses light to produce an enlarged view of an object An example of a simple microscope is a magnifying glass
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What is a compound microscope? Compound Microscope – contains 2 or more objective lenses. – One lens (low power) produces an enlarged image – Another lens (high power) further magnifies the image
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Magnification vs. Resolution Magnification – the enlargement of an image Resolution – how sharp or in focus an object is
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What is an electron microscope? Electron Microscope – can magnify images more than 250,000 X – Uses electron beams rather than light
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A Scanning Electron Microscope
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An ant
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Leaf from a Black Walnut tree
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Pollen
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A moth
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A human skin cell
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A cancer cell
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Centrifugation Centrifugation – a process by which materials suspended in a liquid can be separated from each other based on density
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Microdissection Microdissection – tiny instruments are used to perform various operations on living cells
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Tissue Culture Tissue Culture – the technique of maintaining living cells or tissues in a culture medium outside the body
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Chromatography Chromatography – any technique that separates different substances based on their chemical and physical properties
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Electrophoresis Electrophoresis – the technique for separating substances made up of particles that have an electrical charge
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Spectrophotometry Spectrophotometry – a technique of using light to analyze samples
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