1. Fig. 9-1

2. Are you the “slow-twitch” or “fast-twitch”? 2:15:25 London 2003

3. Florence Griffith Joyner 100M – 10.49s 1988

5. The Race

6. Fast twitch muscle slow twitch muscle

7. What makes these muscle fibers different? The process for making ATP varies Slow fibers do it aerobically (O 2 ) Fast fibers w/o O 2 (anaerobic) # of mitochondrion vary, amt. of myoglobin

8. Efficiency of Cellular Respiration 36-38 ATP per glucose molecule 40% of energy from glucose is harvested 60% heat 1 muscle cell spends/regenerates 10 million ATP/sec

9. Fig. 9-2 Light energy ECOSYSTEM Photosynthesis in chloroplasts CO 2 + H 2 O Cellular respiration in mitochondria Organic molecules + O 2 ATP powers most cellular work Heat energy ATP

11. C 6 H 12 O 6 + 6 O 2  6 CO 2 + 6 H 2 O + Energy Energy = (ATP + heat) Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

13. Fig. 9-UN2 becomes oxidized becomes reduced

14. Fig. 9-UN1 becomes oxidized (loses electron) becomes reduced (gains electron)

15. Oxidation of Organic Fuel Molecules During Cellular Respiration During cellular respiration, the fuel (such as glucose) is oxidized and becomes CO 2, and O 2 is reduced forming H 2 O: Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

16. Fig. 9-UN3 becomes oxidized becomes reduced

17. The Stages of Cellular Respiration: A Preview Cellular respiration has three stages: – Glycolysis (breaks down glucose into two molecules of pyruvate) – The citric acid cycle (completes the breakdown of glucose) or Krebs Cycle – Oxidative phosphorylation (accounts for most of the ATP synthesis) or ETC Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

18. Glucose + oxygen GlycolysisKrebs Cycle Electron Transport Chain Carbon dioxide + water

19. Fig. 9-6-2 Mitochondrion Substrate-level phosphorylation ATP Cytosol Glucose Pyruvate Glycolysis Electrons carried via NADH Substrate-level phosphorylation ATP Electrons carried via NADH and FADH 2 Citric acid cycle

22. Fig. 9-6-3 Mitochondrion Substrate-level phosphorylation ATP Cytosol Glucose Pyruvate Glycolysis Electrons carried via NADH Substrate-level phosphorylation ATP Electrons carried via NADH and FADH 2 Oxidative phosphorylation ATP Citric acid cycle Oxidative phosphorylation: electron transport and chemiosmosis

23. The process that generates most of the ATP is called oxidative phosphorylation because it is powered by redox reactions BioFlix: Cellular Respiration BioFlix: Cellular Respiration Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

24. Fig. 9-7 Enzyme ADP P Substrate Enzyme ATP + Product

25. Concept 9.2: Glycolysis harvests chemical energy by oxidizing glucose to pyruvate Glycolysis (“splitting of sugar”) breaks down glucose into two molecules of pyruvate Glycolysis occurs in the cytoplasm and has two major phases: – Energy investment phase – Energy payoff phase Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

26. Fig. 9-8 Energy investment phase Glucose 2 ADP + 2 P 2 ATPused formed 4 ATP Energy payoff phase 4 ADP + 4 P 2 NAD + + 4 e – + 4 H + 2 NADH + 2 H + 2 Pyruvate + 2 H 2 O Glucose Net 4 ATP formed – 2 ATP used2 ATP 2 NAD + + 4 e – + 4 H + 2 NADH + 2 H +

27. Concept 9.3: The citric acid cycle completes the energy-yielding oxidation of organic molecules In the presence of O 2, pyruvate enters the mitochondrion Before the citric acid cycle can begin, pyruvate must be converted to acetyl CoA, which links the cycle to glycolysis Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

28. The citric acid cycle, also called the Krebs cycle, takes place within the mitochondrial matrix The cycle oxidizes organic fuel derived from pyruvate, generating 1 ATP, 3 NADH, and 1 FADH 2 per turn Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

29. Fig. 9-11 Pyruvate NAD + NADH + H + Acetyl CoA CO 2 CoA Citric acid cycle FADH 2 FAD CO 2 2 3 3 NAD + + 3 H + ADP +P i ATP NADH

30. Fig. 9-16 Protein complex of electron carriers H+H+ H+H+ H+H+ Cyt c Q    VV FADH 2 FAD NAD + NADH (carrying electrons from food) Electron transport chain 2 H + + 1 / 2 O 2 H2OH2O ADP + P i Chemiosmosis Oxidative phosphorylation H+H+ H+H+ ATP synthase ATP 21

31. Fig. 9-14 INTERMEMBRANE SPACE Rotor H+H+ Stator Internal rod Cata- lytic knob ADP + P ATP i MITOCHONDRIAL MATRIX

32. Types of Fermentation Fermentation consists of glycolysis plus reactions that regenerate NAD +, which can be reused by glycolysis Two common types are alcohol fermentation and lactic acid fermentation Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

33. In alcohol fermentation, pyruvate is converted to ethanol in two steps, with the first releasing CO 2 Alcohol fermentation by yeast is used in brewing, winemaking, and baking Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

34. In lactic acid fermentation, pyruvate is reduced to NADH, forming lactate as an end product, with no release of CO 2 Lactic acid fermentation by some fungi and bacteria is used to make cheese and yogurt Human muscle cells use lactic acid fermentation to generate ATP when O 2 is scarce Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings