1. Jason Miller Bethsaida Nieves Biology 240 April 6, 2000
Determining the Effects of Alcohol on the Heart Rate of a 72 Hour Chicken Heart
Jason Miller
Bethsaida Nieves
Biology 240
April 6, 2000

2. Purpose
To study the effects of alcohol on the 72 hour chick embryonic heart, by applying different concentrations of alcohol.

3. Hypothesis
The heart rate of a 72 hour chick embryo will regress due to the amount of the alcoholic solution given to the embryo.

4. 1. Preparation of Dilutions
Methods
1. Preparation of Dilutions
0.4%, 0.2%, 0.02%

5. Methods 2. Windowing an egg - In Vivo (Cruz, 1993)
3. Explanting the egg - In Vitro (Cruz, 1993)

6. Experimental Procedure
12 Embryos
3 Control Embryos
3 Embryos with 0.4%
3 Embryos with 0.2%
3 Embryos with 0.02%

7. Alcoholic Effects
Alcohol is a depressant. Depressants are known to have retardant effects on heart rate and the CNS. However, alcohol is very sporadic at times, and can also increase heart rate. (Logue,1986)

8. TABLE ONE Embryo # In vivo heart rate In vitro Heart rate with alcohol
1- Control
NONE %
3- 0.2%
4- 0.4%

9. * The average of each heart rate is graphed.
Data Results Table 1
* The average of each heart rate is graphed.
Dark Blue: 0.02% Light Blue: 0.2% Gray: 0.4%

10. TABLE TWO Embryo # In vivo heart rate In vitro Heart rate with alcohol
1- Control
NONE %
3- 0.2%
4- 0.4%

11. * The average of each heart rate is graphed.
Data Results Table 2
* The average of each heart rate is graphed.
Dark Blue: 0.02% Light Blue: 0.2% Gray: 0.4%

12. TABLE THREE Embryo # In vivo heart rate In vitro Heart rate
with alcohol
1- Control
NONE %
3- 0.2%
4- 0.4%

13. * The average of each heart rate is graphed.
Data Results Table 3
* The average of each heart rate is graphed.
Dark Blue: 0.02% Light Blue: 0.2% Gray: 0.4%

14. Conclusion
The heart rate of each experimental embryo decreased compared to the first in vivo heart rates that were taken. The application of alcohol resulted in an even lower heart rate. As previously explained, alcohol is a depressant. However, it may be very sporadic at times, and can also increase heart rate, therefore, our hypothesis was not supported.

15. Conclusion
Heart rate: After windowing the egg, the heart rate decreased due to lack of warmth from the goose neck lamp. After explanting, the heart rate decreased even more because of the temperature of the warm chick saline solution.

16. Future Experiments
Apply the solutions while in vivo rather then in vitro.
Apply the same concentrations (0.02%, 0.2% and 0.4%) to a more developed embryo.
Apply different concentrations to the 72 hour chick embryo.

17. REFERENCES
Cruz, Y.P Laboratory Exercises In Developmental Biology. Academic Press, San Diego, California, 241 pages. [ISBN ] [book].
Logue, A.W The Psychology Of Eating And Drinking. W.H. Freeman and Company, New York, 298 pages.