1. Diffusion

3. Selectively Permeable Membrane

4. Animal Cell (Normal Saline 0.9%) = solute

5. Diffusion experiment Methylene blue MW 320g/mole Potassium permagnate MW 150 g/mole Form Hypothesis T 0 initial diameter T 60 final diameter Activity 1 Room temp Calc. rate of diffusion

6. TimeMB RTPP RT T010 mm T6020 mm 10 mm/hr Table 1. Rate of Diffusion in Methylene Blue vs Potassium Permagnate at Room Temp

7. Table 2. Rate of Diffusion in Methylene Blue vs Potassium Permagnate at Room Temp Group Data GroupMB RTPP RT 110mm/hr8 mm/hr 2 3 4 5 Ave SD

8. Create 4 dialysis bags, filled with unknown concentration sucrose solutions (10 ml) Weigh bags and then immerse in 10% sucrose solution (50 ml) Reweigh bags after 30 minutes of experiment Identify the bags containing 0%,10%, 20%, and 40% of sucrose, based on weight changes. Activity 2 Osmosis through semipermeable membrane

9. Unknown solutions of sucrose (0, 10, 20 & 40 %)

10. Dialysis Tubing 1.Open tubing with water 2.Tie knot at one end 3.Fill with one of the unknown solutions (10ml) 4.Tie other end

11. Weigh pre and post

12. 50 ml 10% sucrose Unknown solution (10ml) (0, 10, 20 & 40 % sucrose solution) 30 minutes