2. Lesson 14a: DNA Structure and Replication Trans – carrysoma - body Scrib – writegen - origin Co – togetherre - again

3. A single DNA molecule can be more than a meter long! So, how do you pack it into the nucleus of one tiny cell? A single DNA molecule can be more than a meter long! So, how do you pack it into the nucleus of one tiny cell? DNA folding http://www.dnatube.com/video/47/3D- structure-of-chromosome DNA folding http://www.labaction.com/view_video.ph p?viewkey=f0af142b6d3bead98a92 DNA Folding (Best) 2:14 http://www.cells.de/cellseng/1medienarchi v/Zellstruktur/Zellkern/DNA_condensation/ Flash__C13105.htm

4. Copying DNA A dividing cell duplicates its DNA – creates 2 copies of all DNA – separates the 2 copies to opposite ends of the cell – splits into 2 daughter cells  But the DNA starts loosely wound in the nucleus  If you tried to divide it like that, it could tangle & break nucleus cell DNA

5. Organizing & packaging DNA nucleus cell DNA nucleus cell DNA in chromosomes in everyday “working” cell DNA in chromosomes in cell getting ready to divide DNA has been “wound up”

6. How many chromosomes do you see? KARYOTYPE = display of chromosomes laid out in pairs from largest to smallest. Sex cells are always placed at the end. N o w, h o w m a n y c h r o m o s o m e s d o y o u s e e ? I s t h i s a m a l e o r f e m a l e ? I T ’ S A G I R L !

7. How many chromosomes? Which gender (sex)? It’s a BOY!

8. Paired bases DNA structure – double helix 2 sides with rungs in the middle - like a twisted ladder Bases match together – A pairs with T A : T – C pairs with G C : G

9. DNA Replication Matching bases allows DNA to be easily copied

10. Copying DNA – making exact copies – Replicate = to copy – DNA starts as a double-stranded molecule matching bases (A:T, C:G) – Bonds between A:T and C:G are weak DNA Replication

11. Strands “unzip” at the weak bonds between bases DNA Replication

12. DNA polymerase Enzyme – DNA polymerase – adds new bases Free floating DNA bases in nucleus

13. DNA Replication Get 2 exact copies of DNA to split between the two new cells. DNA polymerase DNA polymerase Coach L14

14. Copying & packaging DNA Copying DNA Coil DNA into compact chromosomes When cell is ready to divide… – copy DNA first, then… – coil up doubled chromosomes like thread on a spool… now can move DNA around cell without having it tangle & break

15. double-stranded human chromosomes ready for mitosis

16. TESTTIPS Make sure you do everything in your power to plan your schedule wisely the day before the test. Plan to get to bed early so you will be well rested for the day of the test.