1. Cellular Respiration Cellular Respiration: Harvesting Chemical Energy
Ppt from: aurumscience.com

2. Life Requires Energy Living cells require energy from outside sources
Some animals, such as the giant panda, obtain energy by eating plants; others feed on organisms that eat plants

4. Energy flows into an ecosystem as sunlight and leaves as heat
Photosynthesis uses sunlight to generate oxygen and glucose sugar.
Cell respiration uses chemical energy in the form of carbohydrates, lipids, or proteins, to produce ATP.

5. ATP ATP stands for Adenosine Tri-Phosphate
ATP is a molecule that serves as the most basic unit of energy
ATP is used by cells to perform their daily tasks

6. ATP
ATP can be broken down into a molecule of ADP by removing one of the phosphate groups.
This releases energy.
ADP can be remade into ATP later when the cell has food that can be broken down (i.e. glucose)

7. NADH
NADH is a molecule that can “carry” H+ ions and electrons from one part of the cell to another.
NADH is the “energized” version of this molecule that is carrying the H+ ion and two high-energy electrons.
NAD+ is the “non-energized” version of this molecule that does not have the ion or the extra two electrons.

8. Adenosine triphosphate (ATP)
LE 8-9 P P P
Adenosine triphosphate (ATP)
H2O P + P P +
Energy i
Inorganic phosphate
Adenosine diphosphate (ADP)

9. LE 9-2 Light energy ECOSYSTEM Photosynthesis in chloroplasts CO2 + H2O
Simple sugars
(Glucose)
+ O2
Cellular respiration
in mitochondria
ATP
powers most cellular work
Heat
energy

10. Cell Respiration and Production of ATP
The breakdown of organic molecules (carbohydrates, lipids, proteins) releases energy.
Cellular respiration consumes oxygen and organic molecules and yields ATP
Although carbohydrates, fats, and proteins are all consumed as fuel, it is helpful to trace cellular respiration with the sugar glucose:
C6H12O6 + 6O2  6CO2 + 6H2O + Energy

11. Glycolysis Glycolysis is the first stage of cellular respiration.
Occurs in cytoplasm.
During glycolysis, glucose is broken down into 2 molecules of the 3-carbon molecule pyruvic acid.
ATP and NADH are produced as part of the process.

12. ATP Production
2 ATP molecules are needed to get glycolysis started.

13. ATP Production
Glycolysis then produces 4 ATP molecules, giving the cell a net gain of +2 ATP molecules for each molecule of glucose that enters glycolysis.

14. NADH Production
During glycolysis, the electron carrier 2 NAD+ become 2 NADH.
2 NADH molecules are produced for every molecule of glucose that enters glycolysis.

15. Glycolysis Glycolysis uses up: Glycolysis produces
1 molecule of glucose (6-carbon sugar)
2 molecules of ATP
2 molecules of NAD+
Glycolysis produces
2 molecules of pyruvic acid (3-carbon acids)
4 molecules of ATP
2 molecules of NADH

16. Advantages of Glycolysis
Glycolysis produces ATP very fast, which is an advantage when the energy demands of the cell suddenly increase.
Glycolysis does not require oxygen, so it can quickly supply energy to cells when oxygen is unavailable.

17. Movement to the Citric Acid Cycle
Before the next stage can begin, pyruvic acid must first be transported inside the mitochondria.
Pyruvic acid is combined with an enzyme called Coenzyme A. This enzyme helps with the transportation.
Pyruvic acid + Coenzyme A make Acetyl CoA
One more molecule of NADH is produced.
This also releases one molecule of CO2 as a waste product.

18. LE 9-10
CYTOSOL
MITOCHONDRION
NAD+
NADH
+ H+
Acetyl Co A
Pyruvate
CO2
Coenzyme A
Transport protein

19. Krebs Cycle
During the citric acid cycle, pyruvic acid produced in glycolysis is broken down into carbon dioxide and more energy is extracted.

20. Citric Acid Cycle
Acetyl-CoA from glycolysis enters the matrix, the innermost compartment of the mitochondrion.
Once inside, the Coenzyme A is released.

21. Citric Acid Cycle
The molecule of acetate that entered from glycolysis joins up with another 4-carbon molecule already present.
This forms citric acid.

22. Citric Acid Cycle
Citric acid (6-carbon molecule) is broken down one step at a time until it is a 4-carbon molecule.
The two extra carbons are released as carbon dioxide.

23. Citric Acid Cycle
Energy released by the breaking and rearranging of carbon bonds is captured in the forms of ATP, NADH, and FADH2.
FADH2 has the same purpose as NADH – to transport high-energy electrons and H+ ions.

24. Citric Acid Cycle
For each turn of the cycle, the following are generated:
1 ATP molecule
3 NADH molecules
1 FADH2 molecule

25. Citric Acid Cycle
Remember! Each molecule of glucose results in 2 molecules of pyruvic acid, which enter the Krebs cycle.
So each molecule of glucose results in two complete “turns” of the Krebs cycle.
Therefore, for each glucose molecule:
6 CO2 molecules,
2 ATP molecules,
8 NADH molecules,
2 FADH2 molecules are produced.

26. LE 9-11 Pyruvic acid (from glycolysis, 2 molecules per glucose) CO2
Citric
acid
cycle
Oxidation
phosphorylation
NAD+
CoA
NADH
ATP
ATP
ATP
+ H+
Acetyl CoA
CoA
CoA
Citric
acid
cycle 2
CO2
FADH2
3 NAD+
FAD 3
NADH
+ 3 H+
ADP + P i
ATP

27. Electron Transport Chain
The electron transport chain occurs in the inner membrane of the mitochondria.
Electrons are passed along the chain, from one protein to another.
Each time the electron is passed, a little bit of energy is extracted from it.
Electrons drop in energy as they go down the chain and until they end with O2, forming water

28. Electron Transport Chain
NADH and FADH2 pass their high-energy electrons to electron carrier proteins in the electron transport chain.

29. Electron Transport Chain
At the end of the electron transport chain, the electrons combine with H+ ions and oxygen to form water.

30. Electron Transport Chain
Energy generated by the electron transport chain is used to move H+ ions (from NADH and FADH2) against a concentration gradient.
This creates a “dam” of H+ ions in the outer fluid of the mitochondria.

32. The electron transport chain generates no ATP
The chain’s function is to break the large free- energy drop from food to O2 into smaller steps that release energy in manageable amounts.
The end result is a “reservoir” of H+ ions that can be tapped for energy, much like a reservoir in a hydroelectric dam.

33. Chemiosmosis
The electron transport chain has created a high concentration of H+ ions in the outer fluid of the mitochondria.
H+ then moves back across the membrane, into the inner fluid.
H+ ions pass through a channel protein called ATP Synthase
ATP synthase uses this flow of H+ to convert ADP molecules (low energy) into ATP (high energy)

34. LE 9-14 INTERMEMBRANE SPACE H+
A rotor within the membrane spins as shown when H+ flows past
it down the H+ gradient. H+ H+ H+ H+ H+ H+
A stator anchored in the membrane holds the knob stationary.
A rod (or “stalk”) extending into the knob also spins, activating catalytic sites in the knob. H+
Three catalytic sites in the stationary knob join inorganic phosphate to ADP to make ATP.
ADP +
ATP P i
MITOCHONDRAL MATRIX

35. Total ATP Production
During cellular respiration, most energy flows in this sequence:
glucose  NADH 
 electron transport chain chemiosmosis ATP
About 40% of the energy in a glucose molecule is transferred to ATP during cellular respiration, making about 38 total ATP
Remainder is lost as waste heat

36. Fermentation Cellular respiration requires O2 to produce ATP
Glycolysis can produce ATP with or without O2 (in aerobic or anaerobic conditions)
In the absence of O2, glycolysis can couples with a process called fermentation to produce ATP.

37. Types of Fermentation
Fermentation consists of glycolysis + reactions that regenerate NAD+, which can be reused by glycolysis
Two common types are alcohol fermentation and lactic acid fermentation

38. Alcohol Fermentation
Yeast and a few other microorganisms use alcoholic fermentation that produces ethyl alcohol and carbon dioxide.
This process is used to produce alcoholic beverages and causes bread dough to rise.
Pyruvic acid + NADH → Alcohol + CO2 + NAD+

39. Lactic Acid Fermentation
Most organisms, including humans, carry out fermentation using a chemical reaction that converts pyruvic acid to lactic acid.
Pyruvic acid + NADH  Lactic acid + NAD+

40. In lactic acid fermentation, pyruvate is reduced to NADH, the only end product is lactic acid. No carbon dioxide is released.
Lactic acid fermentation by some fungi and bacteria is used to make cheese and yogurt
Human muscle cells use lactic acid fermentation to generate ATP when O2 is scarce (out of breath)
Result: Soreness!

41. Yeast and many bacteria are facultative anaerobes, meaning that they can survive using either fermentation or cellular respiration
Most other organisms cannot survive in the long-run using glycolysis and fermentation, they require oxygen.
These are obligate aerobic organisms.

42. LE 9-18
Glucose
CYTOSOL
Pyruvate
No O2 present
Fermentation
O2 present
Cellular respiration
MITOCHONDRION
Ethanol or
lactate
Acetyl CoA
Citric
acid
cycle

43. The Evolutionary Significance of Glycolysis
Glycolysis occurs in nearly all organisms
Glycolysis probably evolved in ancient prokaryotes before there was oxygen in the atmosphere

44. Other Energy Sources
Catabolic pathways funnel electrons from many kinds of organic molecules into cellular respiration
Glycolysis accepts a wide range of carbohydrates
Proteins must be digested to amino acids; amino groups can feed glycolysis or the citric acid cycle
Fats are digested to glycerol (used in glycolysis) and fatty acids (used in generating acetyl CoA)
An oxidized gram of fat produces more than twice as much ATP as an oxidized gram of carbohydrate

45. LE 9-19 Carbohydrates Fats Amino acids Sugars Glycerol Fatty acids
Proteins
Carbohydrates
Fats
Amino
acids
Sugars
Glycerol
Fatty
acids
Glycolysis
Glucose
Glyceraldehyde-3- P
NH3
Pyruvate
Acetyl CoA
Citric
acid
cycle
Oxidative
phosphorylation

46. Video Review on ATP & Respiration