1. Final stuff: n Lab practical: Apr 29 n Final exam: due Fri May 7 @ 2:15

2. Today n IDEM sampling n Assessment exercise

3. MACROINVERTEBRATE COMMUNITY MONITORING EXAMPLE INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT

4. STAGES OF BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

5. What are We Trying to Measure ??

6. “The ability of an aquatic ecosystem to support and maintain a balanced, integrated, adaptive community of organisms having a species composition, diversity, and functional organization comparable to the best natural habitats within a region.” BIOLOGICAL INTEGRITY

7. STAGES OF A BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

8. Aquatic Larvae Aquatic Adults Terrestrial Adults

9. Adult and Larval Caddisflies (Trichoptera)

10. USEPA Rapid Bioassessment Protocols Riffle Habitats

11. Two Composite KICK Screen Samples

12. STAGES OF A BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

13. Sample Elutriation  Remove Gravel and Sand  Remove Excess CPOM

14. The Final Sample Ready for Preservation

15. Field Preservation of Samples

16. GPS for Spatial Reference PAPERWORK…PAPERWORK…. TRIPLE REDUNDANCY  Water Chemistry  Habitat Assessments  Field Observations

17. STAGES OF A BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

18. All Samples are Returned to Laboratory for Processing

19. Squares (25 cm 2 ) Removed by Suction to Petri Dish 100 Organism Sub-Sample 15 Minute Sort Sub-Sample

20. 100 Organism Sub-Sample Selected at Random Second Sub-Sample 15 Minutes Entire Pan “Randomly” Sorted Using Forceps Final Sort Under Microscope

21. Sub-Samples Processed to Family Level

22. All Samples Preserved for Permanent Museum Storage

23. STAGES OF A BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

24. TEN SUB- METRICS SITE INFORMATION TAXONOMIC INFORMATION AND TAXA COUNTS LABORATORY BENCHSHEET

25. Example of 2 Sub-Metrics E P T EPT Index = 6 Families EPT Count = 7 Individuals Metric Scores = 0, 2, 4, 6, 8

26. Scoring Criteria for Riffle KICK Samples

27. Not All Rivers and Streams Have Riffle Habitats

28. Multi-Plate Samples are Collected Individually Multi-Plate Samples are Preserved in the Field and Scraped in the Laboratory

29. Systematic Community Assessments of Benthic Macroinvertebrate Communities are Effective SUMMARY

30. IBI -- Family Level Multi-Metric Indices Provide: n n Cost Effective Data of Ecological Health of Rivers and Streams n n n n Assessment Information to Make Management Decisions

31. Lammert & Allen 1999 Scoring Classification Metric135 Total Taxa< 2223 - 30> 30 Mayfly Taxa0 - 34 - 6> 6 Caddisfly Taxa2 - 34 - 5> 5 Dipteran Taxa3 - 45 - 6> 6 Relative Abundance of Omnivores 70 – 100 %57 – 69 %43 – 56 % Relative Abundance of Filterers 51 – 69 %50 – 31 %9 – 30 % Relative Abundance of Grazers 0 – 19 %20 – 32 %> 33 % Relative Abundance of Predators 24 – 33 %14 – 23 %2 – 13 % Dominance68 – 88 %47 – 67 %24 – 46 %

32. Actual Observation IBI Score (no.) Total Taxa 191 Mayfly Taxa 21 Caddisfly Taxa 21 Dipteran Taxa 31 Relative Abundance of Omnivores 0.425 Relative Abundance of Filterers 0.025 Relative Abundance of Grazers 0.011 Relative Abundance of Predators 0.431 Dominance 0.305 B-IBI score Lammert & Allan (1999)Total IBI21