1. Breed population analyses: some background, a guide to the reports, & some interesting examples.

2. Inbreeding defined as the mating of genetically related individuals. Inbreeding

3. 2 n ancestors where n = generations back We all have 2 parents 4 grand parents 8 great grand parents 16 great great grand parents etc…

4. 10 generations ago was the 1700s mainly isolated rural communities How many of us have 1024 unrelated ancestors from this era?

5. 25 generations ago was the 1300s To be completely non-inbred we would need over 33½ million unrelated ancestors

6. Furthermore, the resemblance of relatives… means selection will result in the mating of individuals more closely related than average

7. Inbreeding defined as the mating of genetically related individuals. So, inbreeding is impossible to avoid (to some degree & if you go back far enough) However, most of the problems associated with inbreeding come from more recent ancestral generations – why? Inbreeding

8. Why? Inbred lines tend to die out… time

9. It’s all about risk…

10. Alvarez et al (2009) PLoS ONE 4(4): e5174 What are the risks associated with inbreeding?

11. Why does inbreeding cause these problems? CGT-CGT-CGT-CGT-TGACGT-CGT-CGT-CAT-TGACGT-CGT-CGT-CGC-TGA

12. CGT-CGT-CGT-CGT-TGACGT-CGT-CGT-CAT-TGACGT-CGT-CGT-CGC-TGA Why does inbreeding cause these problems?

13. COI is a probability that the 2 copies of a gene are Identical By Descent (IBD) 25% for offspring of a full sib mating or a parent/offspring mating 12.5% for offspring of a half sib mating 6.25% for offspring of 1st cousins Coefficient of inbreeding (COI)

14. Alvarez et al (2009) PLoS ONE 4(4): e5174 COI = 25.4%

15. Breedmean COI (2014 born dogs) German Shepherd Dog 3.2% Norwegian Elkhound 4.4% Cavalier King Charles Spaniel 5.5% Labrador 6.5% Bloodhound 7.0% Tibetan Terrier 7.8% Welsh Corgi (Cardigan) 9.9% English Springer Spaniel 9.9% Manchester Terrier 15.1% Breed average COI

16. COI = 0% * *…(using 3 generations of pedigree only, actual COI may be considerably higher. When using 5 generations of pedigree, COI = 17%) Number of generations is all important

17. 20% 15% 10% 5% Average COI Generations / time Rate of inbreeding (ΔF) rate of inbreeding (ΔF) = change in average COI over time (or generations) Usually quoted as the Effective population size (Ne)

18. Pr = 0.06 Pr = 0.0004 f(g) = 0.05f(g) = 0.15 Pr = 0.045 Pr = 0.0003 f(g) = 0.30 (1 in 20 green)(3 in 20 green)(6 in 20 green) (6/100) (45/1000) (4/10,000) (3/10,000) Genetic drift

20. Inbreeding/drift summary Inbreeding unavoidable in the long term COI describes the probability of IBD, and so risk Rate of inbreeding the important measure for breed/population Inbreeding and drift act on allele frequencies

21. Paper detailing results now available: http://www.cgejournal.org/

22. Breed analysis reports

23. Figure 1: registrations by year of birth Trend of registrations over year of birth (1980-2014) = 194.77 per year (with a 95% confidence interval of 143.33 to 246.21)

24. Table 1: sire statistics per year

25. Figure 2: rate of inbreeding Estimated effective population size= 81.7 NB - this estimate is made using the rate of inbreeding over the whole period 1980-2014

26. Table 2: trends within 1980-2014

27. Figure 3: distribution of progeny per sire/dam

28. Broad trends and interesting examples…

29. The rate of inbreeding is (generally) slowing…

30. …although, not in all breeds…

31. Some rarer breeds are conserving diversity…

32. …but others are really struggling…

33. Drastic loss of genetic diversity in some breeds

34. Effective and actual pop n size appear unrelated

35. Possible effect of ‘sub’ populations…

36. Summary General trend across breeds is a declining rate of inbreeding Effective population size is over whole period 1980-2014 Be aware of changing trends within the whole period No simple relationship between actual and effective population size

37. Thank you – and any questions? With grateful acknowledgements to: Dr Sarah Blott – University of Nottingham Prof John Woolliams – Roslin Institute Dr Tom Lewis Tom.Lewis@thekennelclub.org.uk