1. Respiratory System Diffusion Xinping Yue xyue@lsuhsc.edu Department of Physiology LSUHSC-NO

3. Definition of Diffusion of a Gas Net movement of the molecules of a gas from a region of higher concentration to a region of lower concentration. Or Net movement of the molecules of a gas from a region in which it exerts a higher partial pressure to an area in which it exerts a lower partial pressure.

4. Linear Velocity = Flow / Cross sectional Area (cm/sec)(cm 3 /sec) (cm 2 )

5. 1.Diffusion through the gas phase 2.Diffusion through alveolar- capillary membrane (Fick’s Law) 3.Diffusion through the plasma 4.Combine with hemoglobin

6. The Laws Governing the Behavior of Gases  Henry’s Law : The amount of a gas absorbed by a liquid with which it does not combine chemically, is directly proportional to the partial pressure of the gas to which the liquid is exposed and its solubility in the liquid. The solubility of CO 2 in the liquid phase is ~24 times that of O 2.  Graham’s Law : The rate of diffusion of a gas (in the gas phase) is inversely proportional to the square root of its molecular weight.  Fick’s Law of Diffusion : A x D x (P 1 – P 2 ) T V gas =.  MW of CO 2 MW of O 2 Diffusion rate for O 2 Diffusion rate for CO 2 = 1.17

7. Fick’s Law for Diffusion V gas = A x D x (P 1 – P 2 ) T T V gas = volume of gas diffusing through the tissue barrier per time (ml/min) A = surface area available for diffusion (70 m 2 in a healthy adult) D = diffusion coefficient, or diffusivity, of the particular gas P 1 – P 2 = partial pressure difference of the gas across the barrier T = thickness of the barrier or the diffusion distance (0.2 to 0.5  m).. D  solubility MW CO 2 is diffuses ~20 times (24/1.17) more rapidly through the alveolar-capillary barrier than O 2

8. Limitation of Gas Transfer Diffusion-limited, CO Perfusion-limited, N 2 O Perfusion-limited, O 2

9. Limitation of O 2 Transfer Healthy at rest – perfusion-limited Healthy during excise – may reach diffusion limitation Abnormal alveolar-capillary barrier – diffusion limited

10. Limitation of CO 2 Transfer Healthy at rest – perfusion-limited Healthy during excise – may reach diffusion limitation Abnormal alveolar-capillary barrier – diffusion limited

11. Diffusing Capacity V x = A x D x (P 1 – P 2 ) T T VxVx VxVx A x D T T. (P 1 – P 2 ). = =(DL x ) = = VxVx VxVx (P Ax – P Cx ) DL x. ml / min / mm Hg Fick’s equation:

13. Measurement of Diffusing Capacity = = V CO (P A CO – P C CO ) DL CO = = V CO (P A CO – 0).. = = V CO P A CO.

14. Conditions that Decrease the Diffusing Capacity Thickening of the barrier – Interstitial or alveolar edema – Interstitial or alveolar fibrosis Sarcoidosis Scleroderma Decreased surface area – Emphysema – Tumors – Low cardiac output – Low pulmonary capillary blood volume Decreased uptake by erythrocytes – Anemia – Low pulmonary capillary blood volume Ventilation-perfusion mismatch

15. Normal Lung IPF Lung

16. Emphysema