1. Lab 27 Goals and Objectives: **NO DISINFECTING, NO HAND WASHING before 33&36!!!** Exercise 31: Lethal Effect of UV Light Score plates (+++, ++, +, -) for growth using control side of each plate as +++ for comparison; record on class sheet Exercise 33: Effectiveness of Alcohol as an Antiseptic Each person do, follow supplemental packet directions p.113 Exercise 36: Effectiveness of Hand Scrubbing Four groups total: each group elect a scrubber and scrubber assistant, Others do plating; Use solid pre-poured plates, plate using flame sterilized spreader, 10 plates per group: 1 tap water control (0.4ml), 3 basin A (0.1ml, 0.2ml, 0.4ml) 3 basin B (0.1ml, 0.2ml, 0.4ml), 3 basin C (0.1ml, 0.2ml, 0.4ml) Exercise 34: Antimicrobial Sensitivity Testing Four groups: each test all 8 antibiotics against one organism (assigned), two plates per organism, 4 discs per plate. Exercise 35: Evaluation of Antiseptics Each try five agents against both organisms S. aureus & P. aeruginosa SAME five agents for both to compare Gram pos. vs. Gram neg.

2. Effectiveness of Alcohol as an Antiseptic Divide plate into 6 wedges AB C D E F A = left/non-dominant thumb dirty B = left/non-dominant thumb again (how many microbes removed just by touching agar?) C = right/dominant thumb dirty D = right/dominant thumb dipped in alcohol and allowed to dry (how many microbes removed/killed by alcohol dip? E = right/dominant index finger F = right/dominant index finger wiped with alcohol swab (how many microbes removed/killed by swabbing with alcohol?) Replacement Directions for Exercise 33 Supp. page 125

3. Fig. 36.1 Stop at step 5 while you still have skin!

4. 10 plates total: 1 tap water control (0.4ml), 3 basin A (0.1ml, 0.2ml, 0.4ml) 3 basin B (0.1ml, 0.2ml, 0.4ml) 3 basin C (0.1ml, 0.2ml, 0.4ml)

5. Exercise 34: 2 plates each organism, 4 discs per plate Each organism gets tested against 8 different antibiotics Group 1 Staphylococcus aureus Group 2 Escherichia coli Group 3 Proteus vulgaris Group 4 Pseudomonas aeruginosa

6. Lab 27 Goals and Objectives: **NO DISINFECTING, NO HAND WASHING before 33&36!!!** Exercise 31: Lethal Effect of UV Light Score plates (+++, ++, +, -) for growth using control side of each plate as +++ for comparison; record on class sheet Exercise 33: Effectiveness of Alcohol as an Antiseptic Each person do, follow supplemental packet directions p.113 Exercise 36: Effectiveness of Hand Scrubbing Four groups total: each group elect a scrubber and scrubber assistant, Others do plating; Use solid pre-poured plates, plate using flame sterilized spreader, 10 plates per group: 1 tap water control (0.4ml), 3 basin A (0.1ml, 0.2ml, 0.4ml) 3 basin B (0.1ml, 0.2ml, 0.4ml), 3 basin C (0.1ml, 0.2ml, 0.4ml) Exercise 34: Antimicrobial Sensitivity Testing Four groups: each test all 8 antibiotics against one organism (assigned), two plates per organism, 4 discs per plate. Exercise 35: Evaluation of Antiseptics Each try five agents against both organisms S. aureus & P. aeruginosa SAME five agents for both to compare Gram pos. vs. Gram neg.