1. Production of Erythropoietin in Pichia Pastoris
Eporis – Team 15
Team
Background
Problem
Design Alternatives
Design Norms

2. Team Eporis Abby Leistra Stephen Tubergen Zion Lee Nick Giles Team
Background
Problem
Design Alternatives
Design Norms
1/7

3. Background: What is Erythropoietin?
Erythropoietin (EPO) is a glycoprotein hormone
Promotes red blood cell production
Naturally made in kidneys
Treats anemia caused by
Chronic kidney disease
Cancer chemotherapy
HIV therapy
$13 Billion Market
glycam.org
Team
Background
Problem
Design Alternatives
Design Norms
2/7

4. Problem Expression: Chinese Hamster Ovary (CHO) Cells
An eight-week treatment can cost up to $11000
Well studied
Expensive feed
Slow growth rate
Team
Background
Problem
Design Alternatives
Design Norms
3/7

5. Solution? Expression: Pichia Pastoris cells Relatively new
Cheap feed (methanol)
Fast growth rate
2013.igem.org
Team
Background
Problem
Design Alternatives
Design Norms
4/7

6. Design Alternatives Pichia Pastoris Strain Erythropoietin Structure
Turn on and off right genes
Erythropoietin Structure
Glycosylation and
other modifications
glycam.org
Team
Background
Problem
Design Alternatives
Design Norms
5/7

7. Design Alternatives Bioreactors Separation Units Team Background
Mianbin Wu, et. al. (2011). DOI: /20246.
Bioreactors
Separation Units
Team
Background
Problem
Design Alternatives
Design Norms
6/7

8. Design Norms Stewardship Transparency Trust Team Background Problem
Design Alternatives
Design Norms
7/7

9. Questions?