1. Mouse Models of Prostate Cancer Name: Vicki Javier Mentor: Todd Hricik Institution: MSKCC

2. Prostate Cancer: disease marked by abnormal growth of tumor cells in the prostate gland Prostate Cancer is detected by –Digital Rectal Exam –Biopsy –PSA serum reading Prostate Cancer

3. PSA is a biomarker for CaP Our Question: Does PSA play a role in CaP? -Mouse studies PSA Serum Screen

4. PSA mouse study Mice don’t have PSA We already put WT PSA gene into mice We now need a mutant form of PSA gene to put in mice so that we can compare whether the mut mouse will develop CaP in a different way than the WT PSA mouse

5. Mutate the PSA gene (in a plasmid) nucleotide sequence so that a Serine amino acid is changed to an Alanine in the PSA protein (gene product) Prepare the mutated PSA gene for injection into the mouse Project Goal

6. SDM Site Directed Mutagenesis TCT = Serine GCT = Alanine

8. Tools

10. Restriction Digestion of DNA Direction of DNA travel 6000 bp 5000 bp 250 bp

11. References Hricik, Todd http://www.web- books.com/MoBio/Free/Ch9G.htmhttp://www.web- books.com/MoBio/Free/Ch9G.htm http://en.wikipedia.org/wiki/Site- directed_mutagenesishttp://en.wikipedia.org/wiki/Site- directed_mutagenesis http://www.ehealthmd.com/library/prostater emoval/PR_glossary.htmlhttp://www.ehealthmd.com/library/prostater emoval/PR_glossary.html

12. Acknowledgements Todd Hricik Dr. Sat Bhattacharya Sven Ross Neil