2. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA’s Earth Science Enterprise (ESE) Chuck Trees and Jason Perl Center for Hydro-Optics and Remote Sensing (CHORS) San Diego State University (ctrees@chors.sdsu.edu) (jperl@chors.sdsu.edu)

3. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) EOS Terra & Aqua Data Products Program - 3,000 samples/yr Augmentation for an additional 1,800/yr = (4,800/yr) 1. Van Heukelem and Thomas (2001) Filtration 1. GF/F glass fiber filters (25 mm) 2. Positive pressure filtration 3. Filtration Volumes a. Oligotrophic waters - 3-4 liters b. Mesotrophic waters - 1-2 liters c. Eutrophic waters - 0.5-1 liter HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V)

4. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) Sample Handling, Storage and Shipping 1. Fold filters in half with filtered sides facing in. 2. Place in aluminum foil envelops, cryogenic vials or HistoPrep tissue capsules. 3. Label samples containers with a sequential number 4. Place and ship (dry shipper) in liquid nitrogen (LN 2 ). Recordkeeping (electronically transmitted to CHORS). 1. For each sample record volume filtered, station number, date, time, depth, lat and long. HPLC Phytoplankton Pigment Protocols

5. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) Template for Ancillary Data

6. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) HPLC System Calibrations 145 pigment compound calibration curves with 6-10 injections per curve (870-1,450 std injections or 24-40 days of continuous running standards).

7. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) Fluorometer Calibration

8. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) Number of Samples by Date and PI.

9. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) Number of Samples Analyzed by PI

10. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) Number of Samples Pending

11. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) Vitamin E Internal Standard (284 nm) Zeaxanthin Divinyl Chl b Divinyl Chl a Monovinyl Chl a Vitamin E  Carotene Problems - Vitamin E Internal Standard trans-  -apo-8'-carotenal (Fluka Chemical Company)

12. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) 1. Mixing the sample with the buffer. Heukelem and Thomas (2001) method picks up in a ‘train’ (150-75-75-75-150). It is mixed when injected onto the column. CHORS HPLC System mixes in a 2nd vial. Minimized the mixing time, but the uncertainty increased 3-4%. 2. UV/VIS halogen lamp. Faulty connection at the base of the lamp which would cause erratic readings. Solution was to have several lamps in stock to test when the lamp failed. Other Problems with HPLC Method and System

13. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) Culture Mixture (450 nm) Chl c 3 Injection Spike Peridinin Alloxanthin Fucoxanthin .  carotene Monovinyl Chl a Diatoxanthin 19’-Hexanoyloxyfucoxanthin Zeaxanthin Monovinyl Chl b Monovinyl Chls a’, a” Chl c 2 Diadinoxanthin Prasinoxanthin .  carotene Mixture of Pigments from Cultures

14. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) SeaHARRE-3 Analysis

15. MODIS Science Team Meeting Baltimore, MD (4-6 Jan 06) “To Separate or Not to Separate? That is the HPLC Question: A comparison between C 18 and C 8 methods” J. Perl, Q. Williams, and C. Trees “Particulate absorption: a pigment reconstruction analysis” Q. Williams, J. Perl, and C. Trees AGU/ASLO/TOS Ocean Sciences Meeting (Honolulu, 20-24 Feb 06)