Presentation on theme: "A Model of Non-Enzymatic Nucleic Acid Elongation and Replication. Chrisantha Fernando*, Eors Szathmary **, Johan Elf o *Center for Computational Neuroscience."— Presentation transcript:
A Model of Non-Enzymatic Nucleic Acid Elongation and Replication. Chrisantha Fernando*, Eors Szathmary **, Johan Elf o *Center for Computational Neuroscience and Robotics, Dept. of Informatics, University of Sussex, Falmer, Brighton BN1 9RH, UK and Collegium Budapest (Institute for Advanced Study), Szenth á roms á g u. 2, H-1014 Budapest, Hungary **Dept of Plant Taxonomy and Ecology, E ö tv ö s University, and Collegium Budapest (Institute for Advanced Study), Szenth á roms á g u. 2, H-1014 Budapest, Hungary o Dept. of Cell & Molecular Biology, Molecular Biology Programme, Biomedical Center, Box 596, Husarg. 3, SE-751 24 Uppsala, Sweden
How did Long Template Replication Originate? The origin of template replication is a major unsolved problem in science. Gantis Hypothesis: They originated in a Protocell. -Autocatalytic Formose Metabolism. -Autocatalytic Membrane System. -Autocatalytic Template Polymerisation. Gantis computational model of template replication was unrealistic, assuming an initiation and a propagation reaction for template growth. If nucleotide like molecules were produced by a cellular metabolism, what would really happen to them?
The Replicase Ribozyme No Replicase Ribozyme has been found, designed, or artificially selected. How could a replicase ribozyme evolve? By natural selection acting on long RNA sequences. But replication is a prerequisite for evolution. Therefore another non-enzymatic means of long nucleic acid replication is needed.
Long Templates Have Been Synthesized. Non-enzymatic synthesis of templates up to 55 nucleotides has been achieved on mineral surfaces, but there is no replication, because templates do not recycle by unzipping.
Short Templates Can Replicate. Short oligonucleotide analogues can self- replicate, but longer ones cannot because self-inhibition by strand association becomes prohibitive.
Our Findings. We identify four main obstacles to the replication of longer strands.
Competition by successfully unzipping short replicators.
Premature detachment of incomplete copies from longer strands
Elongation v. Replication. We show in silico that at low temperatures and high polymer concentrations, the first two obstacles are avoided, allowing unlimited elongation by association of staggered duplex oligomers. However, low temperatures magnify the last two obstacles.
Previous Models. An chemical kinetic model predicted replication at low temperature.
What is a Stochastic Model? Represent an Integer number of molecules in a fixed volume reactor. For each possible reaction in the reactor, calculate a propensity (rate*activity), and generate a time according to the distribution t i = -log(rand()/p i ). Execute the reaction with the earliest t i. Update only the propensities that have been affected by the executed reaction.
What are Non-Uniform Cellular Automata? state neighborhood In uniform cellular automata each cell obays the same transform- ation rules. In a NUCA, the transformation rules depend on the cell state.
What are Disjoint Cellular Automata? Polymer 1 Polymer 2 Polymer 3 Polymer 4 Each polymer in Its own CA. Association rules determine inter- actions between CAs.
Let the CA represent 2 types of activated monomers (nucleotides) with properties resembling nucleic acids. Able to make strong covalent bonds along the chain (P-bonds). Able to undergo base pairing (H-bonds) between opposite monomers. The H-bonds and P-bonds are the primitives of the stochastic model.
VERY SLOW Spontaneous Formation of P-bonds: we assume it is so slow that we do not model it. A much more likely way for a p-bond to form is shown next……
QUITE SLOW P-bonds can form between nucleotides, on another p-bond opposite.
P-bonds also break. More easily on single strands than double strands.
Template Directed P-bond formation rate. P-bond degradation rate. P-bond formation and breakage are functions of temperature. temperature Rate
The chance of the first h-bond being formed between two polymers depends on the product of available h-bond sites on those two polymers. e.g. 6 * 9 for the two polymers above. Polymer A. Polymer B. Only associations that conform to the 2D grid topology are permitted.
Arbitrary configurations in the 2D grid topology are possible. E.g. Many will be very unstable and only form transiently….
Others will be more stable… We do not predefine the expected configurations in the effectively infinite space of all possible configurations. They arise due to the underlying intra- and inter- polymer dynamics I have described.
Two Time-Scale Dynamics P-bonds form and break much slower than H-bonds. Run H-bond dynamics to equilibrium. Sample microstates at equilibrium. Calculate p-bond formation and breakage propensities for each microstate, and execute a p-bond event.
Control Experiments Rare configurations will be under-represented due to the small numbers of polymers simulated. Melting Temperature curves could be reproduced.
Results Initialized with 50 ds 6-mers (0.0016M), and 20 monomers (10 A, and 10 B) (0.0006M). 280K, 7.8 years. Longest polymer length 103.
Mechanism of Elongation can be Directly Observed.
Elongation Occurs only at Low Temperatures. - Same conditions as before. - 121 hours. - 320K.