1. California Science Standards #1d,4c,5a,5b,6b,6c,7c DNA: Replication Copy the Blueprints

2. 2 DNA: Replication Before a parent cell divides into two identical daughter cells (mitosis), it must copy its instruction booklet (DNA) Making an exact copy of the DNA prior to cell division is called REPLICATION  See video, “DNA_replication,” © Glencoe, 2000 http://www.wiley.com/college/pratt/0471393878/student/animations/dna_replication/index.html

3. 3 DNA Replication 3 easy steps:  1. DNA unwinds (“unzips”)  2. Free nucleotides attach  3. Two copies of DNA result

4. 4 Replication- Step 1 1. DNA unwinds (“unzips”)  Helicase enzymes separate the two strands of DNA Note: if a word ends with “ase”, it is an enzyme (a protein catalyst, a helper molecule)  Hydrogen bonds between base pairs (A-T, G-C) are broken  The point of separation between strands is the “replication fork” http://www.wiley.com/college/pratt/0471393878/student/animations/dna_replication/index.html

5. 5 Replication- Step 2 2. Free nucleotides attach  DNA polymerases bind to the separated strands of DNA  A polymerase “hooks up” free-floating nucleotides to their complements on the single strand (A~T, C~G) Covalent bonds are formed within the backbone (between sugar and phosphate) Hydrogen bonds are formed with the other nitrogenous bases (between strands) http://www.mcb.harvard.edu/Losick/images/TromboneFinald.swf

6. 6 Replication- Step 3 3. Two copies of DNA result  Each new copy of DNA is not totally new Each has one strand from the old DNA This is called “semi-conservative” replication http://www.stolaf.edu/people/giannini/flashanimat/mol genetics/dna-rna2.swf

7. 7 Complementary Base Pairing  Problem: Predict the sequence of nucleotides on the new strand, given the sequence on one of the old strands of DNA TAAGCGA ATTCGCT

8. 8 Practice What will the new strand look like? GTAACGC ???????

9. 9 Answer What will the new strand look like? GTAACGC CATTGCG

10. 10 Quick & Accurate DNA polymerases begin replication simultaneously at many points (thousands) along the separated chain  Broken zipper analogy Remarkable accuracy: only one error made for every 10,000 base pairs (99.99% accurate)  These errors can be fixed by DNA “proofreader” enzymes and other means  After fixing, accuracy jumps to one error for every 1,000,000,000 base pairs (99.9999999% accurate)!

11. 11 Accidents Happen With Some “Accidents” (Base Mismatches) Leading to Mutation A mutation is a heritable change in DNA sequence. Mutations due to replication errors only happen once in every billion replicated nucleotides.

12. 12 Mutation Some mutation is good, too much is bad. The effects of mutation are usually bad or neutral - only sometimes are mutations beneficial. Cells employ elaborate mechanisms to prevent mutation – but the mechanisms aren’t perfect. Mutations are the root cause of cancer (bad). Mutations are the only way to introduce novel alleles into a species (good for evolution). So, just like Goldilocks – not to hot, not too cold, just right – the optimal rate of new mutation is a balancing act.

13. 13 DNA Damage is Often the Root Cause of Mutation DNA may be chemically altered (i.e. damaged) spontaneously and by chemicals and radiation. DNA Damage is Often the Root Cause of Mutation

14. 14 Mutation as a Villain Cancerous growths that result from loss of a protein that polices DNA for errors.

15. 15 Cancer Incidence Increases Sharply with Age The increase is due at least in part to the age-related accumulation of multiple mutations in single cells.

16. 16 Poor Little Froggies  Problem: Populations of Cascade frogs and western toads (Oregon Cascade Range) are in decline  Hypothesis: Ozone depletion  increased UV radiation  increased mutations  decrease in population in small critters like frogs  Data: Pacific tree frog populations NOT declining  Data: Pacific tree frogs’ DNA repair enzymes better able to repair mutations than those of CF and WT  Conclusion: ?

17. 17 Poor Little Froggies  Problem: Populations of Cascade frogs and western toads (Oregon Cascade Range) are in decline  Hypothesis: Ozone depletion  increased UV radiation  increased mutations  decrease in population in small critters like frogs  Data: Pacific tree frog populations NOT declining  Data: Pacific tree frogs’ DNA repair enzymes better able to repair mutations than those of CF and WT  Conclusion: DNA repair enzymes from CF and WT unable to keep up with mutations