1. How is DNA used to solve crimes? 8 th Grade Forensic Science T. Trimpe http://sciencespot.net/

2. What is DNA? DNA stands for deoxyribonucleic acid and contains genetic information. It is found on chromosomes located in the nucleus of our cells and makes each person unique. DNA Image: http://science.howstuffworks.com/genetic-science/dna-evidence.htmhttp://science.howstuffworks.com/genetic-science/dna-evidence.htm What makes up DNA? The sides or backbone of the DNA molecule are made up of sugar (deoxyribose) and phosphate molecules. The rungs that form the middle of the molecule are made up of pairs of nucleotides or nitrogen bases. Adenine (A) pairs with thymine (T), while guanine (G) always pairs with cytosine (C). The order of the bases determines the genetic code. Double Helix

3. Where is DNA found in the body? DNA is contained in blood, semen, skin cells, tissue, organs, muscle, brain cells, bone, teeth, hair, saliva, mucus, perspiration, fingernails, urine, feces, etc. Approximately 98% of our DNA code as humans is the same in everyone. The portion of DNA that is similar in all humans is not important in forensic analysis. It is the other 2% of our DNA software that is our biochemical profile and therefore most important to forensic investigators. What is the DNA extraction procedure? After a sample of DNA has been collected, the DNA is extracted from each cell. Specific regions of the DNA molecule are amplified in a process called PCR (Polymerase Chain Reaction) that was developed in 1987. The PCR process is very rapid and therefore a great advantage in an ongoing case. PCR is an improvement over previous processes because even if only a very small sample is left at the crime scene, it can still be used.

4. Reading a DNA sample All United States forensic laboratories now use PCR-based DNA testing featuring STR (Short Tandem Repeat) analysis. STRs are short fragments of “junk” DNA that are generally located between genes and vary in length. Currently 13 STR sites are used. Once a sample has been amplified and the STR sites are chosen, the sample is analyzed. Depending on how quickly the segments of DNA move through the analysis, a “peak and valley” type graph is printed. Each peak location shows the number of times a specific STR is repeated at a particular site. Since chromosomes are in pairs (one from Mom and one from Dad) you could actually have two peaks for each site. If there is only one peak, it would be homozygous (meaning the same) for that site. If there are two different peaks, it would be heterozygous (meaing different) for that site.

5. How is DNA used as evidence? Each person’s DNA is different from other people (except identical twins). DNA collected from a crime scene can either link a suspect to the evidence or eliminate a suspect, similar to the use of fingerprints. DNA can identify a victim through DNA from relatives, even when no body can be found. DNA can link crime scenes together by linking the same perpetrator to different scenes locally, statewide, and across the nation. DNA can place an individual at a crime scene, in a home, or in a room where the suspect claimed not to have been. DNA can refute a claim of self-defense and put a weapon in the suspect's hand. It can change a story from an alibi to one of consent. DNA Strand Image & information : http://www.dna.gov/audiences/investigators/know/http://www.dna.gov/audiences/investigators/know/

6. What factors affect DNA evidence? Several factors can affect the DNA left at a crime scene, such as environmental factors (e.g., heat, sunlight, moisture, bacteria, and mold). Therefore, not all DNA evidence will result in a usable DNA profile. Further, DNA testing cannot identify when the suspect was at the crime scene or for how long. DNA information : http://www.dna.gov/audiences/investigators/know/http://www.dna.gov/audiences/investigators/know/ CODIS stands for COmbined DNA Index System, which is an electronic database of DNA profiles that can identify suspects. DNA profiles from individuals convicted of certain crimes, such as rape, murder, and child abuse, are entered into CODIS and help officers identify possible suspects when no prior suspect existed. What is CODIS? Did you know? Each human cell contains three billion DNA base pairs. Our unique DNA amounts to 0.1% or 3 million base pairs. Watch the video segment from NOVA: "The Killer's Trail" and be ready to answer the questions on the next slide.

7. Video Quiz Choose the best answer for each. 1. Who was the victim? A. Marilyn SheppardB. Sam SheppardC. Sam Sheppard, Jr. 2. What are the keys to DNA fingerprinting? A.ChromosomesB. AllelesC. Nitrogen bases 3. Where did the scientist get the sample of DNA for Marilyn Sheppard? A. HairB. SkinC. Fingernail 4. Whose blood was found in the blood trail? A. Marilyn SheppardB. Sam SheppardC. Neither

8. DNA Analysis Site Number EvidenceSuspect 1Suspect 2Suspect 3Suspect 4Suspect 5 1 st #2 nd #1 st #2 nd #1 st #2 nd #1 st #2 nd #1 st #2 nd #1 st #2 nd # 1 2 3 4 5 6 7 8 9 10 11 12 13

9. True or False? Which three statements below are true? 1. The DNA in a man's blood is the same as the DNA in his skin cells and saliva. 2. Each person's DNA is different from every other individual's. 3. DNA can be found in all the cells in our bodies except the blood cells. 4. DNA can have forensic value even if it is decades old. 5. DNA evidence was first used to get a conviction in a trial in 1987. Watch the video segment from NOVA: "The Killer's Trail" and be ready to answer the questions on the next slide.