1. Studi di immunogenicità in modelli murini Laura Pisapia Giovanna Del Pozzo Napoli, 27 Giugno 2013

2. Corso C. et al. EBP1 and DRBP76/NF90 binding proteins are included in the Major Histocompatibility Complex Class II RNA operon. 2011 N.A.R Sep 1;39(16):7263-75. Pisapia L. et al. Co-regulated expression of alpha and beta mRNAs encoding HLA-DR surface heterodimers is mediated by the MHCII RNA operon. 2013 N.A.R. Apr1;41(6):3772-86. MHC class II RNA Operon

3. EBP1 Identified as ErbB3 binding protein, is the human homologue of mouse cell-cycle regulated protein p38-2G4 In carcinoma cell lines p48 isoform functions as tumor suppressor In PC12 and glioblastoma p48 works as oncogene, while p42 isoform is a tumor suppressor Translational control: in the cytoplasm is associated to 80S ribosomes and polysomes inhibits translation of AR mRNA Transcriptional repressor: Inhibits transcription of E2F regulated promoters by recruiting HDAC2/Rb/Sin3A comple x RNA binding protein: influences bcl2 stability, promotes decay of androgen receptor mRNA, binds UTRs of MHCII p48 and p42 isoforms

4. p48 isoform binds 5’ and 3’UTR of MHCII mRNAs The over expression of p48 isoform determines an up- regulation of MHC Class II in different cancer cell lines Over-expression of p42 and p48 EBP1 influences MHCII amount

5. OT-II MOUSE OT-I MOUSE Spleen T CD4+ magnetic selection T CD8+ magnetic selection B16-OVAa and CD8+ co-culture B16-OVA and CD4+ co-culture Melanoma B16-OVA CD8+ T cell CD4+ T cell CD8 OVA 257–264 (SIINFEKL) peptide CD4 OVA 323–339 (ISQAVHAAHAEINEAGR) peptide CD8+ CFSE labelling CD4+ CFSE labelling T cells activation and proliferation are demonstrated by the decrease of CFSE labelling CFSE