1. LEARNING OBJECTIVES 1. Overall objectives - Principles that underlie different electrical recording techniques - Physiological and biophysical information the techniques provide 2. Extracellular recording and multi-electrode arrays - spiking (all-or-none) information, neural codes conveyed by individual neurons and by groups of neurons 3. Intracellular recording - measurements of input resistance, synaptic input, and synaptic integration 4. Patch-clamp recording (cell-attached; whole-cell; inside-out patch; outside-out patch) - measurements of input resistance, synaptic input, synaptic integration; characteristics of voltage-gated ion channels and single ion channel events Biophysics 6702 Patch Clamp Techniques Stuart Mangel, Ph.D.

2. EXTRACELLULAR VS. INTRACELLULAR RECORDING Extracellularly and intracellularly recorded voltages are in the microvolt and millivolt ranges, respectively.

3. Maintaining the resting membrane potential V m = ln RT F p K [K + ] o + p Na [Na + ] o + p Cl [Cl - ] i p K [K + ] i + p Na [Na + ] i + p Cl [Cl - ] o The Goldman-Hodgkin-Katz (GHK) Equation: The steady state membrane potential for a given set of ionic concentrations inside and outside the cell and the relative permeability of the membrane to each ion extracellular intracellular E Na = +56 Na + (150) E K = -102 K + (3) E Cl = -76 Cl - (120) E Ca = +125 Ca 2+ (1.2) Na + (18)K + (135) Cl - (7) Ca 2+ (0.1 µM) Na +,K + -ATPase -60 to -75 mV NSCC

4. Measuring E M Measure the potential difference between two electrodes using a D.C. amplifier Expected value of the membrane potential is in millivolts (not microvolts), so the gain does not need to be as high INTRACELLULAR RECORDING

5. Intracellular Recording When a fine-tipped electrode penetrates the membrane of a cell, one observes a sudden change in the measured potential to a more negative value. Typical problems –High impedance μE –Damage when cell penetrated

6. Wheatstone Bridge Used to measure an unknown resistance Discovered by Hunter Christie, 1833 Popularized by Charles Wheatstone MEASURING THE INPUT RESISTANCE

7. BALANCING THE BRIDGE R1 = Fixed R R2 = Variable R R3 = Fixed R R4 = Unknown R To get R2/R1 = R4/R3, adjust R2, so that there is no current across B, C R4 = (R2/R1)·R3

8. CALCULATING THE INPUT RESISTANCE OF A CELL Balance the bridge before entering the cell After impaling the cell, the bridge is “out of balance” by the R value of the cell I is known, measure V, and calculate R using Ohm’s Law (V = IR) R = V/I “Balanced”“Out of Balance” Did R increase or decrease? Did channels open or close?

9. PATCH-CLAMP RECORDING Neher and Sakmann, Nobel Prize, 1991 Tremendous technical breakthrough that improved the signal to noise ratio of electrical recordings Record from whole cells or from a small patch of cell membrane, so only a few ion channels (or one) can be studied High resistance (in giga-ohms) and high mechanical strength of the seal between the glass electrode and the cell membrane enable one to observe very small currents. The diameter of the tip of patch electrodes can be larger than that of fine-tipped intracellular microelectrodes (1.0 micron vs. 0.05 microns), so that the resistance of patch electrodes is lower (e.g. 5 MΩ vs 200 MΩ). The lower resistance of patch electrodes makes voltage clamping easier.

10. Patch clamp recording configurations Electrode Glass pipette Ion channel Plasma membrane Cell-attached Inside-out Outside-out Whole-cell suction pull Perforated-patch antibiotics

11. SUMMARY OF ADVANTAGES AND DISADVANTAGES OF PATCH CLAMP CONFIGURATIONS

12. THE VOLTAGE CLAMP

13. THE ACTION POTENTIAL

14. Voltage clamping reveals the ionic currents that underlie the action potentials observed in squid axons

15. Activation and Inactivation Properties Ionic Selectivity

16. Reversal potentials for synaptic currents

17. Inhibitory actions of GABA synapses result from the opening of ion channels selective for Cl -

18. SODIUM CHANNEL CURRENTS RECORDED FROM CELL-ATTACHED PATCH

19. Properties of ACh- gated channels

20. Single open ACh-gated channels behave as simple resistors.

21. Extracellular Mg 2+ ions block NMDA channels under physiological conditions.

22. SUMMARY OF ADVANTAGES AND DISADVANTAGES OF PATCH CLAMP CONFIGURATIONS

23. Questions: Stuart Mangel, Ph.D. Professor Department of Neuroscience The Ohio State University College of Medicine 614-292-5753 mangel.1@osu.edu Readings: Kandel, Schwartz et al., Principles of Neural Science, 2013, 5 th Ed., Chap. 7, 9, 10 Squire, Berg et al., Fundamental Neuroscience, 2008, 3 rd Ed., Chap. 6, 11