1. LOC Design with CAD Lecture Slides

2. Learning Objectives  Learn creating LOC part file using Solidworks  Learn to dimension the LOC part file

3. Lab-on-a-Chip Designs  For this class you will create two LOC designs per team.  The two designs are created to adapt your design ideas to two different chipholder designs  4-Hole Chipholder  3-Hole Chipholder  The project description document defines the requirements of the two designs (Project Description Document section – Chip Requirements)

4. LOC in Chipholder

5. Leveraging Lab 1 & 2 Experiences  What went wrong or did not work well  How can this “generic benchmark” be improved upon, given the problems?  What prevents good performance (e.g., dirt, scratches, poor seals, channel/well proximity, well geometry, etc.) and how can this knowledge be used to create a better design and better practices when testing the design?  Use your observations! Be creative!  Your design should be a marriage of function and form!

6. Wafer Manufacturing Constraints  Wafer designs will be milled from an acrylic material  The milling machine will be using a 0.010”circular bit.  Should not create any internal sharp corners since a circular bit cannot make that shape.  For such features, round the internal corners with a radius of curvature of at least 200 microns.  Also no channel or opening can be smaller than 300 microns.  Extruded part file should be 1/8” thick with wells and channels 200 microns deep. Put your group letter on one side of the chip for identification.

7. Round the internal corners with a radius of curvature of at least 200 μm

8. Deliverables Design Deliverables Each team will provide two chip designs for the LOC device – One design for each chipholder Initial Sketch Design Submission for the 1 st chip Preliminary 1 st design 1st Chip Design Submission 2 nd Chip Design Submission Hand-drawn sketchRequired Not required Solidworks DeliverableNot required Dimensione d Part File to GTA Dimensioned Part File to GTA 1.Printout of dimensioned drawings 2. Send Solidworks part file to GTA Operational ProcessNot required Per Project Desc. Doc. Revised CalculationsNot required Detection well Volume Per Project Desc. Doc.

9. Preliminary 1 st Design  Solidworks Part File emailed to GTA, due per website.  Includes: Use of 4-hole chip holder design (seed file) to ensure well alignment  Dimensioned Part File  Calculation of detection well volume  Include separate simple logo to identify your chip  Instructional Team will provide feedback of the designs

10. 1 st Design Submission  Solidworks Part File emailed to GTA, due per website.  Includes: Use of 4-hole chip holder design (seed file) to ensure alignment.  Operational Process per Proj. Desc. Doc.  Calculations for Detection Well as per Proj. Desc. Doc.  Instructional Team will provide feedback on the designs

11. 2 nd Design Submission  Based on the revisions suggested in the 1st design Should be drawn on 3 hole chip holder design (seed file) to ensure alignment.  Dimensioned Chip Design with top view scaled up to 3:1.  Send the part files (.SLDPRT) to TA (insure proper extrusion)  Revised Operational Process  Calculations for all features as per Proj. Des. Document

12. Part file (.SLDPRT) Submission  Send one part file per design to your GTA  CONVERT UNITS TO INCHES  USE FILE NAMING CONVENTION FOR EACH FILE  EXAMPLE: “LOC_SP15_B_DESIGN1.SLDPRT” LAB ON CHIP Semester And Year GROUP CHIP NUMBER

13. LOC Specifications  Chip Diameter – 50800 μm  Chip Thickness – 5000 μm  Depth of ALL Features – 200 μm  Channel widths – 300 μm – 400 μm  Detection Well Volume – 3 μl  Round all sharp corners!  Refer to Project Description Document section – Design Deliverables for more details

14. Solidworks Part File  Use the 4 Hole (1 st Design) and 3 Hole (2 nd Design) chipholder templates to create your design.  Please use the seed files for the chip designs available on the course website.

15. Generic Dimensioned Design

16. Chip Holder Design with Chip

17. Solidworks Drawing Steps  If not already open, open your part file for your Design (LOC_SP13_A_DESIGN1.SLDPRT). Select “Drawing” from the upper left- hand new file icon.  Click on Model View on the Drawing Toolbar  In the property manager, under Part/Assembly to Insert, select the file. Click next. Under orientation, click “front” under standard views.  Under Scale, select custom scale, User Defined, and set to 3:1.  DO NOT include the chip holder in this layout.

18. Dimensioning in Solidworks  Under Annonate, choose Smart Dimension to dimension each feature of the drawing.  Choose the standard OSU title block. Fill out the title block.  Resize and format the text in the view for proper visibility as necessary.  Save using the file naming conventions and save as type “Drawing Files (*.SLDDRW)”.