1. Parvovirus B19 Genotype 2 Plasma, Sourced from the US Dr Sally A. Baylis, Division of Virology NIBSC

2. Identification of Parvovirus Genotype 2 Plasma Plasma obtained at NIBSC in 2000, as a diluent for validation of HCV RNA assays Plasma obtained at NIBSC in 2000, as a diluent for validation of HCV RNA assays Tested by conventional PCR using 3 sets of primers of B19V (2002) Tested by conventional PCR using 3 sets of primers of B19V (2002) Retested in error in 2004, by a consensus real-time PCR for parvovirus B19 – unexpectedly high titre Retested in error in 2004, by a consensus real-time PCR for parvovirus B19 – unexpectedly high titre DNA sequence analysis confirmed that presence of gt2 B19V (Accession # EF216869) DNA sequence analysis confirmed that presence of gt2 B19V (Accession # EF216869) 98.29% nt identity vs IM-81 (4904 bp) 98.29% nt identity vs IM-81 (4904 bp) 97.83& nt identity vs A6 (4846 bp) 97.83& nt identity vs A6 (4846 bp) This plasma was sourced in the US; it is not a single unit This plasma was sourced in the US; it is not a single unit

3. Genotype 2 Titre The plasma has a titre of 6.2 log 10 IU/ml The plasma has a titre of 6.2 log 10 IU/ml Agreement between consensus in-house assay & Artus RealArt TM Parvo B19 LC kit) Agreement between consensus in-house assay & Artus RealArt TM Parvo B19 LC kit) No amplification plot in Roche assay No amplification plot in Roche assay 330 mls of the plasma are available 330 mls of the plasma are available Plasma pools associated with this sample has a B19V loads between 7.6-8.5 log 10 IU/ml Plasma pools associated with this sample has a B19V loads between 7.6-8.5 log 10 IU/ml Products associated with the pools contain a mixture of gt1 & gt2 B19V Products associated with the pools contain a mixture of gt1 & gt2 B19V No B19V infectivity can be found in the gt2 plasma No B19V infectivity can be found in the gt2 plasma