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Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007.

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Presentation on theme: "Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007."— Presentation transcript:

1 Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007

2 University of MN Department of Horticulture

3 2,4-dichlorophenoxyacetic acid (2,4-D) http://files.agrichem.net/agrbv/artinfo/foto.org/2,4,%20d%20Amine%20a.JPG

4

5 Background Light intensity and hue angle decreases (Rosen, et al.)

6 Components of Color Light intensity Chroma (saturation) Hue angle www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf

7 Components of Color www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf Light intensity Chroma (saturation) Hue angle

8 Components of Color www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf Light intensity Chroma (saturation) Hue angle

9 Components of Color www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf Light intensity Chroma (saturation) Hue angle

10 Background Light intensity and hue angle decreases (Rosen, et al.) Coloration affects sales (Fults, et al.)

11 Background Light intensity and hue angle decreases (Rosen, et al.) Coloration affects sales (Fults, et al.) 2,4-D harmful, LD50 of 1500 ppm ( MSDS found at http://fscimage.fishersci.com/msds/96919.htm )

12 Goal To determine visual color, anthocyanin accumulation, and UFGT accumulation differences

13

14 Anthocyanin Biosynthetic Pathway LDOX UFGT leucocyanidin  anthocyanidin  anthocyanin 3-glucoside (Keifenheim, et al.)

15 Hypotheses With 2,4-D application: Increase in UFGT accumulation

16 Hypotheses With 2,4-D application: Increase in UFGT accumulation Change in color (Rosen, et al.)

17 Hypotheses With 2,4-D application: Increase in UFGT accumulation Change in color (Rosen, et al.) Increase in anthocyanin accumulation

18 Methods

19 Colorimetry http://www.recreonics.com/images3/pocket_colorimeter.gif

20 Components of Color Light intensity Chroma (saturation) Hue angle www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf

21 Anthocyanin Accumulation

22 Quantifying UFGT RNA

23 Quantifying UFGT cDNARNA reverse transcription

24 Quantifying UFGT cDNA Amplified UFGT RNA reverse transcription qPCR

25 Results SS

26 Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

27 Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

28 Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

29 Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

30 Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

31 UFGT Field Data

32 UFGT Greenhouse Data

33 Conclusion Visual color significantly changed (light intensity, hue angle)

34 Conclusion Visual color significantly changed (light intensity, hue angle) Anthocyanin accumulation unchanged

35 Conclusion Visual color significantly changed (light intensity, hue angle) Anthocyanin accumulation unchanged UFGT accumulation decreased

36 Future Work Look at petunidin (purple) vs. pelargonidin (red) anthocyanins

37 Acknowledgements Dr. Cindy Tong Harpartap Mann Dr. Carl Rosen Ms. Fruen Team Research

38 Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007

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