1. Fidgety little critters!
Kingdom Protista
Fidgety little critters!

2. What is a Protist? Eukaryotic Mostly single celled Classified by:
Has a nucleus!
Mostly single celled
Classified by:
MOVEMENT
OBTAIN FOOD
Animal-like:
Heterotrophic
Plant-like:
Autotrophic (have chloroplasts)
Fungus-like:
Parasitic
REPRODUCE

3. Animal-like Protists: Sarcodinia
Movement:
By pseudopods:
Little blobs of cytoplasm
Means “Fake Feet”
Consumer Nutrition:
Surround meal and bring into food vacuole
Phagocytosis!
Reproduction:
Binary Fission
Organism:
Amoeba amoeba

4. Amoeba

5. Animal-like Protists: Ciliates
Movement:
By cilia:
Little “hairs” that beat like oars
Consumer Nutrition:
Food is swept in by cilia to GULLET
Contractile vacuoles collect and “spit out” water
Reproduction:
Binary fission AND conjugation
Organism:
Paramecium caudatum, Stentor coeruleus, Blepharisma

6. Paramecium

7. Stentor

8. Plant-like Protists: Flagellates (Volvox)
Colonial organism:
Hundreds of individual cells “live” together
Movement:
Long, whip-like flagella
Whole colony moves together!
Nutrition:
Autotrophic
Contain chloroplasts! (Hence green color!)
Reproduction:
Binary fission

9. Volvox

10. Euglenophytes: The plant-like animal protist
NO CELL WALL:
Has pellicle
MOVEMENT:
TWO flagella
NUTRITION:
Autotrophic (chloroplasts)
Can be heterotrophic when no sunlight!
Red spot helps organism to find sunlight
REPRODUCTION:
By binary fission
ORGANISM:
Euglena

11. Euglena

12. Helpful Diagrams for Lab
Also called eyespot

13. Helpful Diagrams for Lab
Volvox

14. Helpful Diagrams for Lab

15. Helpful Diagrams for Lab
Stentor
Cilia
Mouth
Gullet
Contractile Vacuole
Food Vacuole

16. General tips/tricks for success the next 2 days…
Follow ALL microscope rules/techniques you’ve learned!
Focus on low power first with coarse adjustment.
Then switch to higher powers and use fine adjustment.
DO NOT mix the droppers! Only use Euglena droppers for Euglena, etc.
Use same slide and cover slip and keep washing them off and drying them each time you get a new specimen.
BE CAREFUL around the live specimen! Only ONE person per group should be making the slide. Don’t crowd the area and increase the risk of spilling the specimen!!

17. General tips/tricks for success the next 2 days…
The stentor and amoeba:
They are supposed to look like blobs of cytoplasm.
They are NOT the tiny swimming things that you may see around the non-moving blobs. Those are probably some other protozoa like paramecium.
You SHOULD move the slide around on the stage to “search” for specimen if you don’t see anything at first.
Also, some of them are VERY fast. Sometimes you only get a quick glance as they “swim” by.

18. General tips/tricks for success the next 2 days…
If at first you don’t succeed…
It’s OK to go back and try to make another slide of the organism. It is quite possible to get one that does not have any of the organism in it.
REMEMBER, you only need a few drops from the pipette onto the slide, though.

19. General tips/tricks for success the next 2 days…
The volvox are SOOOO cool! They are my favorite!
You may want to skip to these first just to ensure a good, LIVE, view of them!
You MAY use a textbook for help labeling the diagrams, BUT the best help will be going to my website and using this PowerPoint!!
The questions in the lab are easy and only require you to read the paragraphs about each organism.