1. Chapter 29/30 DNA Mutation and Repair Pages 975-978 AND 1009-1012 All rights reserved. Requests for permission to make copies of any part of the work should be mailed to: Permissions Department, Harcourt Brace & Company, 6277 Sea Harbor Drive, Orlando, Florida 32887-6777 Learning objectives: Understand the following what are the different types of mutation how are mutations introduced into DNA what are the different types of repair machinery how does each repair mechanism function how is human disease associated with DNA repair defects

2. Define a Mutation A mutationA mutation is a stable change in DNA structure heritable Base sequence is changed Mutations are classified into several “types”Mutations are classified into several “types”

3. Molecular Nature of Mutation (what types of mutations arise) Point mutationsPoint mutations (base substitutions): one base substituted for another Transition mutations = A to G or C to T (purine to purine or pyrimidine to pyrimidine) Transversion mutations = purine to pyrimidine or vice versa Can arise from mispairing, insertion of base analogs, or chemical mutagens (nitrous acid, hydroxylamine and alkylating agents)

4. Consequences of Point mutations missenseA missense mutation: This results in one wrong codon and one wrong amino acid.

5. Consequences of Point mutations nonsense stop A nonsense mutation: If the change in the base sequence results in a stop codon, the protein would be terminated at that point in the message.

6. Consequences of Point mutations senseA sense mutation: This occurs when the change in the DNA sequence results in a new codon still coding for the same amino acid.

7. Other types of mutations Insertions and deletionsInsertions and deletions: result in frameshift mutations (less common) Insert a G here

8. DNA Mutation - How? 1) Through mistakes during DNA replication 2) Spontaneous mutations (deamination, depurination that occur naturally) 3) Induced mutations caused by environmental agents (chemical mutagens, UV radiation)

9. Errors Arising Due to base Mispairing During DNA Replication (page 975) Tautomerization 1) Syn vs anti conformation (flipping around glycosidic bind) 2) H 2 O acting as a bridge 3) Bases can mispair during DNA replication through three (quite rare) mechanisms that change the H-bonding properties of the base so it is incorporated incorrectly.

10. Base Analogs Base Analogs may get incorporated during DNA replication = thymine analog gets incorporated during DNA replication opposite an A frequent tautomer change -now pairs with G Compounds that chemically resemble a nucleotide base closely enough that during DNA replication, they can be incorporated into the DNA in place of the natural base.

11. The base analog, 2-AP pairs with T. But 2-AP can also pair with C via a singe H- bond. Therefore, if 2- AP gets in DNA, then upon replication a C is sometimes inserted. Another base analog

12. DNA Mutation - How? - continued 1) Through mistakes during DNA replication 2) Spontaneous mutations (deamination, depurination that occur naturally) 3) Induced mutations caused by environmental agents (chemical mutagens, UV radiation)

13. Spontaneous DNA Mutation Deamination: - C to U (now pairs with T) (100/cell/day) - A to Hypoxanthine (now pairs with C) - G to Xanthine (still pairs with C) Depurination/Depyrimidination: - removes purine base from DNA(10 4 bases /cell/day) or pyrimidine bases (less frequent) Oxygen radical damage -breaks sugar rings

14. Oxidative deamination The primary amino groups of nucleic acid bases are somewhat unstable. They can be converted to keto groups in reactions like the one pictured here: In a mammalian cell, ~100 uracils are generated per cell per day in this fashion. Other reactions include conversion of adenine to hypoxanthine, and guanine to xanthine.

15. Oxidative deamination other examples

16. Spontaneous Base Loss The glycosyl bond linking DNA bases with deoxyribose is labile under physiological conditions. Within a typical mammalian cell, several thousand purines and several hundred pyrimidines are spontaneously lost per cell per day. Loss of a purine or pyrimidine base creates an apurinic/apyrimidinic (AP) site (also called an abasic site)

17. Oxygen radical damage

18. DNA Mutation - How?- continued 1) Through mistakes during DNA replication 2) Spontaneous mutations (deamination, depurination that occur naturally) 3) Induced mutations caused by environ- mental agents (chemical mutagens, UV radiation)

19. Mutation through Exposure to Environmental Agents Chemical mutagens: - Nitrous acid (HN0 2 ) formed from nitrites (in preserved meats) reacting with stomach acid, causes oxidative deamination - Alkylating agents add methyl or ethyl groups to bases and change their pairing properties - Intercalating agents (ethidium bromide, acridine orange) distort the helix causing frameshift insertions or deletions.

20. Nitrous acid Agents causing oxidative deamination Nitrosamines

21. Agents causing alkylation The first report of mutagenic action of a chemical was in 1942 by Charlotte Auerbach, who showed that nitrogen mustard (component of poisonous mustard gas used in World Wars I and II) could cause mutations in cells.

22. Intercalating Agents

23. Mutation through Exposure to Environmental Agents Ultraviolet Radiation: classified in terms of its wavelength UV-C (180-290 nm)--"germicidal"--most energetic and lethal, it is not found in sunlight because it is absorbed by the ozone layer UV-B (290-320 nm)--major lethal/mutagenic fraction of sunlight UV-A (320 nm--visible)--"near UV"--also has deleterious effects (primarily because it creates oxygen radicals) but it produces very few pyrimidine dimers. Tanning beds have UV-A and UV-B.

24. Ultraviolet Radiation: - causes dimers to form between adjacent T bases on a strand of DNA (or between adjacent T-C bases, hence the term pyrimidine dimer) - a cyclobutyl ring links the adjacent T’s - this interferes with the ability of the T’s to base pair to the opposite strand, and blocks DNA replication

25. The most frequent photoproducts are bonds formed between adjacent pyrimidines within one strand The most frequent are CPDs, cyclobutane pyrimidine dimers T-T CPDs are formed most readily, followed by T-C or C-T; C-C dimers are least abundant. CPDs cause a distortion in the DNA chain structure. The two adjacent pyrimidines are pulled closer to each other than in normal DNA. T-T CPD with cyclobutyl ring in blue

26. DNA Repair A fundamental difference from RNA, protein, and lipid All these others can be replaced, but DNA must be preserved We have seen how changes get stably incorporated into DNA How do the mutations get repaired?? E. coli is the model system for understanding different repair mechanisms

27. Direct repair - fixes pyrimidine dimers (UV damage) Excision repair: i) Base Excision Repair (BER) - fixes abnormal bases (uracil, hypoxanthine, alkylated bases) ii) Nucleotide Excision Repair (NER) - fixes large structural changes and helix distortions (pyrimidine dimers, bulky base adducts) Mismatch repair - fixes mismatches Mechanisms of DNA Repair

28. Pyrimidine dimers can be repaired by: DNA photolyase: Uses energy from light absorption Contains chromophores (light absorbing agents) Action spectrum is blue/near UV light range Steps in the repair mechanism: 1) Enzyme recognizes and binds to the damage 2) Light absorption by chromophore converts it to an excited state 3) Chromophore donates an electron to the cyclobutyl dimer 4) Dimer is destabilized and undergoes a series of electron rearrangements which result in monomeric pyrimidines Direct Repair System Repairs damage without removing the damaged base

29. Direct Repair System Repairs damage without removing the damaged base Photolyase contains 2 chromophores that absorb light energy. In all photolyases, one of the chromophores is FADH-, and the other is either methenyl-tetrahydrofolate (MTHF) or 8-hydroxy-5- deazaflavin (8-HDF). MTHF and 8-HDF act as primary light gatherers (green in the diagram), transferring their energy to FADH- (yellow). The energy from FADH- is used to split the dimer

30. Direct Repair System Repairs damage without removing the damaged base CPD photolyases are found in bacteria, fungi, plants and many vertebrates, but not in placental mammals. As we shall see, humans use a different repair pathway to fix pyrimidine dimers caused by UV light

31. Excision Repair Excision repair: i) Base Excision Repair (BER) - fixes abnormal bases (uracil, hypoxanthine, alkylated bases) ii) Nucleotide Excision Repair (NER) - fixes large structural changes and helix distortions (pyrimidine dimers, bulky base adducts)

32. Base Excision Repair DNA glycosylaseAbnormal bases are recognized by a set of DNA glycosylase enzymes, each one recognizing a specific abnormal base For example, uracil glycosylase recognizes uracil in DNA After recognizing the damage the DNA glycosylase binds to it and initiates a repair pathway by hydrolyzing off the base

33. A DNA glycosylase binds to damaged base AP endonuclease binds and cleaves the DNA backbone Exonuclease removes a segment of DNA DNAP fills in the gap Ligase seals the nick Cleaves N-glycosidic bond Note that the AP site Is identical to that made by spontaneous depurination or depyrimidination.

34. 1. Removal of the incorrect base by an appropriate DNA glycosylase to create an AP site 2. Nicking of the damaged DNA strand by AP endonuclease upstream of the AP site, thus creating a 3'-OH terminus adjacent to the AP site 3. Excision of the AP site, followed by extension of the 3'-OH terminus by a DNA polymerase Steps in Base Excision Repair

35. Base Excision Repair is not Enough BER can’t deal with all types of damage since it requires a DNA glycosylase to recognize each specific damage. The huge variety of chemical mutagens combined with radiation and oxygen radicals make too many types of damage to each be recognized by a different DNA glycosylase.

36. Nucleotide Excision Repair NERFortunately, a different, more flexible damage repair mechanism called NER has evolved. abnormal structure abnormal chemistryNER machinery uses a limited number of proteins to recognize damaged regions in DNA based on their abnormal structure as well as on their abnormal chemistry, then excises and replaces them.

37. In all organisms, NER has the same steps: Damage recognition Binding of a protein complex at the damaged site Double incision of the damaged strand several nucleotides away from the damaged site, on both the 5' and 3' sides Removal of the damage-containing fragment from between the two nicks Filling in of the resulting gap by a DNA polymerase Ligation

38. Fixes damage causing large distortions in the helical structure (e.g. UV-induced pyrimidine dimers, other base adducts) In E. coli the NER machinery repairing UV damage is a protein complex: excinuclease UvrA + UvrB + UvrC = ABC excinuclease UvrD helicase, DNAP I, and ligase also participate Nucleotide Excision Repair

39. 2 UvrA proteins form a complex with one UvrB protein in an ATP-dependent reaction The complex recognizes UV damage by distortion in the helix The UvrA proteins dissociate from the complex after ATP hydrolysis. This leaves UvrB bound across from the damage Nucleotide Excision Repair in E. coli

40. Now UvrB can recruit UvrC protein to the complex UvrC activates UvrB to nick the DNA 4 ntds 3’ from the pyrimidine dimer Then UvrB activates UvrC to nick the DNA 7 ntds 5’ from the pyrimidine dimer This leaves a fragment of DNA containing the damage that can now be removed Nucleotide Excision Repair in E. coli

41. A helicase, UvrD, uses ATP hydrolysis to power the unwinding of the damaged DNA fragment. This reomoves UvrC The gap in the DNA is now filled in by DNAPI or II, reomving uvrB in the process Finally, DNA ligase seals the nick Nucleotide Excision Repair in E. coli

42. Critical for repairing UV-induced damage (because we don’t do direct repair) The principal is the same as in bacteria: i.e. damage is recognized, an excinuclease makes a gap, DNAP fills the gap But the proteins are different (20-30) Defects in NER proteins cause genetic disorders NER in Humans

43. NER in Humans Xeroderma pigmentosum (XP) Genetic disorder with symptoms: -extreme sensitivity to sunlight (by ~age 2), and >1000X higher risk of skin cancer (by ~age 8) Defect is in repair of UV damage Gene mapping identified several repair proteins (called XP proteins) XP-C and XP-A recognize pyrimidine dimers XP-B and XP-D have helicase activity XP-G and XP-F have nuclease activity

44. NER in Humans XP-C recognizes pyrimidine dimers XP-B and XP-D are helicases that separate the DNA strands around the damage. RPA keeps the strands apart XP-G and XP-F are endonucleases that cut the DNA on either side of the damage XP-A binds to the pyrimidine dimer and helps to recruit other proteins to a complex The cut fragment is removed and the gap is filled in by DNAP  or 

45. Real-world biochemistry In 1997, NASA developed a Prototype UV garment for children with XP, Porphyria and other sun Related Disorders to have a Quality of life and Freedom. This NASA UV Protective Project designed for XP was completed in 1998 and the UV garments are being supplied to children of parents that have requested them.

46. Mismatch Repair mismatchesCorrects mismatches after DNA replication Increases replication fidelity by 10 2 -10 3 fold Mismatch repair systems scan newly- replicated DNA duplexes for mismatched bases PROBLEM: which of the 2 strands has the correct sequence and which has the error?

47. Mismatch Repair parentSince the parent (template) strand should have the correct sequence, repair machinery identifies the parent strand and copies it to correct the mistake methylated strand as parentSince methylation occurs post-replication, repair proteins identify methylated strand as parent, remove mismatched bases on other strand and replace them

48. Steps in Mismatch Repair MutS MutLMutS recognizes mismatches and binds to them. Binding of MutL stabilizes the complex. MutHThe MutS-MutL complex activates MutH, which locates a nearby methyl group and nicks the newly synthesized strand opposite the methyl group. UvrDA helicase (UvrD) unwinds from the nick in the direction of the mismatch, and a single- strand specific exonuclease cuts the unwound DNA the gap is filled in by DNAP III and sealed by DNA ligase.

49. Repair of Mismatch Replication Errors (in E. coli) 1. 2. 3. 4. MutS MutL 1. MutS recognizes mismatches and binds to them. Binding of MutL stabilizes the complex. MutH 2. The MutS-MutL complex activates MutH, which locates a nearby methyl group and nicks the newly synthesized strand opposite the methyl group.

50. Continued from the previous slide 1. 2. 3. 4. UvrD 3. A helicase (UvrD) unwinds from the nick in the direction of the mismatch, and a single- strand specific exonuclease cuts the unwound DNA 4. The gap is filled in by DNAP III and sealed by DNA ligase. Repair of Mismatch Replication Errors (in E. coli)

51. Eukaryotic Mismatch Repair Detailed mechanism is unclear Identification of the parent strand is different Human proteins were found similar in sequence to MutL and MutS called hMutL (MLH) and hMutS (MSH) Defects in either MLH or MLS result in an enhanced susceptibility to cancerDefects in either MLH or MLS result in an enhanced susceptibility to cancer (HNPCC=hereditary non-polyposis colon cancer)

52. We have now finished Chapters 29 and 30 All rights reserved. Requests for permission to make copies of any part of the work should be mailed to: Permissions Department, Harcourt Brace & Company, 6277 Sea Harbor Drive, Orlando, Florida 32887-6777 For next class please read: Chapter 31 Pages 1014-1023