1. WP2-P6-INRA LeafyVeg-12December2007 Activity of the P6 in the frame work of the project: regeneration of 300 lettuce accessions WP 2 – P6 (INRA-France) Objectives for year 1: regeneration of 75 L. sativa

2. WP2-P6-INRA LeafyVeg-12December2007 Work made at INRA in year 1 Regeneration of lettuce accessions Control of the harvested seeds Quality : germination Seed production under insect proof plastic tunnel Storage of seeds in 2 conditions (6° and -18°C) Purity : homogeneity of progeny by observation of morphological type Insect-proof tunnel Control of winter cultivars

3. WP2-P6-INRA LeafyVeg-12December2007 Choice of 300 accessions for regeneration  Old public cultivars  Genotypes from public lab (USDA, US universities,…)  Cultivars authorized by breeding companies  Cultivars received more than 20 years ago without restriction Accessions free for seed distribution  300 accessions multiplied in 2006-2010

4. WP2-P6-INRA LeafyVeg-12December2007 Regeneration of lettuce in 2007 Culture in 2007: 38 accessions (with 2 plants)  Sowing of old seeds23 February  Plantation 10 April  Notation of the minimum descriptors for flowering  Harvesting plant by plant from July to August (10-40 g/plant)  Storage of seed samples at -18°C 7 December (about 2 x 5-6 g/accession) Insect-proof tunnel used for seed production

5. WP2-P6-INRA LeafyVeg-12December2007 Culture in field in 2007 : accessions harvested in 2006 Control in 2007 of harvested seeds  Sowing 16 March  Plantation 12 April  Notation of the minimum descriptors for vegetative characters and picturesMay Culture in tunnel in 2007: winter accessions harvested in 2006 or 2007  Sowing 14 September  Plantation 4 October  Notation of the minimum descriptors for vegetative characters and picturesNovember-December

6. WP2-P6-INRA LeafyVeg-12December2007 Winter tunnel in November 2007 Total  for the LeafyVeg project Each accession: 2 plots of 32 plants Other accessions are recent cultivars 12 batavia  1 31 butterhead  19 8 leaf lettuce  1 1 cos

7. WP2-P6-INRA LeafyVeg-12December2007 Control of winter Butterhead lettuce cv GX535 cv Bremex Old cultivars

8. WP2-P6-INRA LeafyVeg-12December2007 Control of winter Butterhead lettuce cv Octavo (RZ) cv Pegase (Vilmorin) Modern cultivars from breeding companies

9. WP2-P6-INRA LeafyVeg-12December2007 Control of winter lettuce Pictures for accession characterization Batavia VerdieButterhead Magdalena Butterhead Pégase Butterhead Méliès Butterhead Raphaela Butterhead Tekla

10. WP2-P6-INRA LeafyVeg-12December2007  Choice of the 300 accessions for ILDB (sent to Rob)  Regeneration of 42 accessions and storage of the seeds  Control of harvested seeds in open field = 27 open field accessions harvested in 2006 under plastic tunnel = 21 winter accessions harvested in 2006-2007  Notation of minimum descriptors and photos  Translation of the press release for publication in French agricultural journals (in collaboration with Valérie) Conclusion of the year 1 for P6 Activities made in 2007

11. WP2-P6-INRA LeafyVeg-12December2007  Copy in Excel format of the minimum descriptors to include in ILDB (+ some other descriptors and photos?)  Possibility to choose the other free accessions of the lettuce (L. sativa) INRA collection to include in ILDB Conclusion of the year 1 for P6 Work still to be done Activities planned in LeafyVeg project  Complete the passport data and send to Rob Other activities